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HPLC and UHPLC reversed-phase method development - Selecting a reproducible starting point

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Join us on Friday, October 30, to learn about choosing a reproducible starting point for chromatographic method development

22 Oct 2020
Finn Price
Administrator / Office Personnel

Expert insights

Dr. Phil Koerner, Phenomenex
Dr. Phil Koerner, Market Development Manager, Phenomenex

In this SelectScience webinar, we focus on reversed-phase chromatography and best practices for selecting a reproducible starting point for your initial method development.

Dr. Phil Koerner, from Phenomenex, will present tips to help you leverage HPLC and UHPLC column particle morphologies to improve or optimize a specific aspect of your separations. Koerner will also discuss separation tradeoffs such as maximizing the relative speed of analysis of your method, versus the need for increased selectivity or compound retention.

Ways for improving your method's reproducibility through robust method development and techniques to ensure the reproducibility through the lifecycle of the method will also be covered.

Register now

Key learning objectives:

  • Method development optimization for reversed-phase chromatography
  • Impact on chromatographic reproducibility
  • Selectivity and particle morphologies considerations

Who should attend:

  • HPLC/UHPLC-focused method developers
  • Analytical development groups

The live webinar takes place on Friday, October 30, at:

  • 16:00 GMT
  • 17:00 CET
  • 12:00 EST
  • 09:00 PST

SelectScience runs 10+ webinars a month across various scientific topics, discover more of our upcoming webinars>>

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Chromatography Method DevelopmentChromatography method development is the process of designing and optimizing chromatographic techniques to separate and analyze complex mixtures. This includes selecting appropriate stationary and mobile phases, determining flow rates, and optimizing detection methods. Successful method development is essential for high-quality results in fields like pharmaceuticals, food safety, and environmental analysis. Explore chromatography method development tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.HPLC Method DevelopmentIt is important to develop and validate HPLC methods in order to identify, quantify or purify compounds of interest in the most efficient way. Effective method development ensures optimization, quality control and cost effectiveness.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.HPLCHigh-performance liquid chromatography (HPLC) is a technique for separating, identifying, and quantifying components in mixtures. It is widely used in chemical, pharmaceutical, and environmental analysis to ensure purity and identify unknown compounds. Explore the best HPLC systems in our peer-reviewed product directory; compare products, read reviews, and get pricing directly from manufacturers.Reversed-Phase LC

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