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HPLC and UHPLC Methods for Melamine Analysis
6 Mar 2009Emily Marquez-Vega
Publishing / Media
Product news
Grace has developed MS-compatible LC methods for Melamine using an HILIC media platform that can be applied to both traditional HPLC as well as UHPLC systems.
Melamine was analyzed with a standard HPLC system using a 5μm particle HILIC phase packed into a 250 x 4.6mm column. The 1.5μm version of this phase was then packed into a high throughput format conducive to UHPLC and fast LC systems. Both methods deliver excellent linearity and use isocratic elution for fast analysis without the need for re-equilibration.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.