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How to rapidly harvest mammalian cell cultures with body feed filtration

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Join this expert webinar to find out all you need to know about an innovative new method for tackling the first step of mAb purification

29 Aug 2019
Charlie Carter
Life Sciences Editor

Expert insights

Cancer research is an increasingly complex field and in the area of monoclonal antibody (mAb) production, many hurdles can be presented, starting initially with the analysis of a multitude of clones in small volumes. After the identification of interesting candidates, larger cell culture volumes are needed, and the expression levels of mAbs are assessed and maximized in cell line development. While there are many solutions on the market for growing mammalian cell cultures of 15-1,000 mL to high densities in parallel reactors or as shaker flask cultures, their purification can lead to a production line bottleneck. One time-consuming hurdle is the clarification and sterile filtration of a high-density culture before further processing.

In this webinar, Dr. Noushin Delmdahl, head of product management lab consumables at Sartorius Lab Instruments, will present an innovative new method for tackling the first step in mAb purification - body feed filtration. This method of filtration is facilitated by the addition of diatomaceous earth (DE) as a filter aid, and has been used in the plasma and food/beverage industries for years.

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Watch this live webinar if you would like to learn about:

  • Harvesting high-density cell cultures from 15mL-1,000mL in only minutes
  • The application of body feed filtration for fast, clean and reliable filtration of high-density mammalian cell cultures
  • The comparison of body feed filtration with traditional centrifugation and subsequent filtration step in terms of mAb recovery, turbidity of cell culture and charge heterogeneity of the clarified molecules

The webinar takes place on Thursday, September 12, 2019, at:

  • 16:00 BST
  • 11:00 EDT
  • 08:00 PDT
  • 17:00 CEST

All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes.

Scheduling conflict? No matter, register to receive an on-demand link to watch later>>

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Engineered AntibodiesEngineered antibodies are modified to enhance specificity, stability, and functionality for therapeutic and diagnostic applications. These antibodies are central to immunotherapy and biomarker detection. Compare engineered antibody solutions in our directory to find top products and reviews.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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How to rapidly harvest mammalian cell cultures with body feed filtration