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How to Overcome Challenges in the Real-Time PCR Workflow

SelectScience

Book a spot on our upcoming free webinar, where you’ll be given a troubleshooting masterclass in RT-PCR

27 Sept 2018
Frankie MacDonald
Administrator / Office Personnel

Expert insights

Dr. Melanie Jahn, Product Manager, RT-PCR, Analytik Jena AG

Real time PCR, is a standard method in research laboratories, and remains one of the most sensitive techniques in functional genomics. The real-time element provided by this technology, however, poses endless possibilities for operator errors and mistakes to occur. Common challenges faced include assay design, template preparation and reporting or interpretation of real-time data.

Perfect for lab scientists, technicians and managers working with real time PCR on a regular basis, expert Melanie Jahn will deliver a comprehensive guide on how to address some of the most common hurdles encountered during the workflow - offering detailed advice and top tips.

In the Q&A session, you can ask your own troubleshooting questions and have Jahn answer them live.

Register now

After the webinar, you should be able to:

  • Understand how the entire real-time PCR workflow can be improved — from assay design, sample treatment and purification, to RT, qPCR and data evaluation
  • Recognize the new PCR technologies which can help overcome these common troubles and challenges during the workflow

The webinar will broadcast live on Thursday, October 4, at:

  • 16:00 BST
  • 17:00 CEST
  • 11:00 EDT
  • 08:00 PDT

If you can’t make the live event, the webinar will be available to watch on-demand to those that register. Book a slot now! >

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RT qPCRReal-time quantitative PCR (RT-qPCR) is a technique used to measure gene expression and quantify DNA or RNA in real-time. It is essential in genomics, diagnostics, and biotechnology research. Explore RT-qPCR systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.

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