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How to Double Your IDs for Single-Cell Proteomics Using µPAC™

SelectScience

Join this webinar to find out how you can use micro pillar array chromatography columns (µPAC™) to improve the sensitivity of low-input proteomic samples

29 Apr 2019
Charlie Carter
Life Sciences Editor

Expert insights

Recent technological innovations focus on profiling complex systems at the single-cell level. The rapidly advancing field of single-cell proteomics aims at comprehensively quantifying protein expression levels from individual mammalian cells, complementing single-cell transcriptomics and genomics analyses. A single mammalian cell contains about 0.3 pg of protein, which is 1,000 times less than what is commonly used in proteomics analysis. Robust analysis of very low input samples poses one of the key challenges to the development of new mass spectrometry-based proteomic workflows, including sample preparation techniques and instrumentation.

This upcoming SelectScience webinar will explore the potential of newly emerging chromatographic technologies to significantly improve sensitivity for low-input proteomics samples, as well as providing detailed experimental results as evidence.

REGISTER NOW

Watch this webinar to learn about:

  • The principles of micro-chip-based pillar array chromatography columns (µPAC™)
  • The benefits and separation performance of µPAC™ columns for the analysis of complex and limited biological samples
  • How to achieve record protein and peptide identifications data using µPAC™ chromatography for single-cell proteomics in comparison to conventional columns
  • The unrivalled retention time stability features of µPAC™ in comparison to conventional columns
  • The compatibility of the µPAC™ trapping column with the µPAC™ analytical

The live webinar takes place on Monday, May 13, 2019, at:

  • 15:00 BST
  • 10:00 EDT
  • 07:00 PDT
  • 16:00 CEST

Karl Mechtler, head of the VBCF (Vienna BioCenter Core Facilities) mass spectrometer pool and the Research Institute of Molecular Pathology (IMP)/Institute of Molecular Biotechnology (MBA)/Gregor Mendel Institute of Molecular Plant Biology (GMI) mass spectrometry facility in Vienna, Austria, will present record protein identification data generated with PharmaFluidics' µPAC™ columns for single-cell proteomics analysis. Geert Van Raemdonck, global field support expert at PharmaFluidics, will also give an introduction on the principles of micro Pillar Array Columns (µPAC™) and their benefits.

Scheduling conflict? No matter, register to receive an on-demand link to watch later>>

Related products

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µPAC™ Trapping column

PharmaFluidics

Our µPAC™ Trapping column was developed with an identical stationary phase support morphology, matching perfectly the retentive properties of our analytical µPAC™ columns. Effectively desalting and preconcentrating the analytes improves the analytical column lifetime and workflow throughput.

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50 cm µPAC™ column

PharmaFluidics

With an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradi­ent solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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200 cm µPAC™ column

PharmaFluidics

As an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.

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Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry.

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How to Double Your IDs for Single-Cell Proteomics Using µPAC™