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How to design, optimize, and validate multiplex immunofluorescence panels

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Join this webinar to explore the biological and technical considerations when developing, testing, optimizing, and validating multiplex panels

10 Mar 2020
Charlie Carter
Life Sciences Editor

Expert insights

Multiplex immunofluorescence (mIF) combines the spatial information from immunohistochemistry (IHC) with multimarker phenotypes. Recent advances in mIF technology have made it possible for researchers to develop novel custom panels, but additional considerations must be taken into account in order to produce a panel which performs at least as well as IHC on a marker-by-marker basis and avoids any undesirable interactions between detection of the targets and neighboring visible light spectra.

In this webinar, Michael J. Surace, Scientist II at AstraZeneca, will explore the biological and technical considerations when designing a panel, describe a linear process for developing, testing, and optimizing a panel, and provide an approach for technical validation of multiplex panels that detects and addresses known risks.

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Key learning objectives:

  • Learn about key factors to consider when designing a new mIF panel
  • Explore an efficient process for developing and optimizing mIF panels using TSA-linked fluorophores and iterative staining
  • Become familiar with the best use cases and advantages of most common mIF staining and imaging technologies
  • Attain an adaptable scheme for testing and validating a novel mIF panel

The live webinar and Q&A session will take place on Thursday, March 26, at:

  • 16:00 GMT
  • 12:00 EDT
  • 09:00 PDT
  • 17:00 CET

Register to watch the full webinar here>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ImmunologyImmunological techniques measure and characterize immune responses. Immunology kits and analysis systems often use techniques such as ELISA, radioimmunoassay (RIA) and immunodiffusion assays, Immunohistochemistry, and flow cytometry. Immunologists use equipment such as flow Cytometers, plate readers, plate washers and fluorescent microscopes.ImmunofluorescenceImmunohistochemistryImmunohistochemistry (IHC) is a technique used to detect specific proteins in tissue samples by using antibodies that bind to target antigens. IHC is widely applied in pathology, immunology, hematology and cancer diagnostics. Explore IHC tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.Method ValidationMethod validation is the process used to prove that the analytical procedure used for a particular test is capable of providing suitable data.MultiplexingMultiplexing refers to the ability to measure multiple targets or analytes simultaneously in a single experiment. This technique is valuable for high-throughput screening, diagnostics, and complex assays, as it increases efficiency and data quality. Browse our peer-reviewed product directory to find the best multiplexing products, compare tools, check reviews, and get pricing directly from manufacturers.

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How to design, optimize, and validate multiplex immunofluorescence panels