Highly Efficient Assay Kit Detects Protein Interactions by Co-Immunoprecipitation

Dualsystems Biotech AG have launched a new assay kit for the detection of protein interactions by co-immunoprecipitation. Formulated to enable highly efficient antigen immunoprecipitation, the FleX-IP kit enables immunoprecipitation using less than 10 μg of antibody and is compatible for use with a wide range of antibodies. Applications include validation of protein interactions by co-immunoprecipitation & immunoprecipitation of proteins from cells and cell-free reactions.

Designed as a complete solution for immunoprecipitation experiments, the FleX-IP kit includes protein A/G agarose, control mouse IgG, lysis/wash buffer, stock NaCl solution, and SDS-PAGE buffer as well as spin columns and collection tubes. Each kit contains sufficient materials for 40 IP reactions and is optimally suited to confirm protein-protein interactions found in DUALhybrid, DUALmembrane, and DUALhunter screens.

FleX-IP immunoprecipitations are performed using common IP procedures. The specific antibody is added to the sample to form an immune complex which is then added to the protein A/G agarose. The complex is washed to remove unbound material and the bound material eluted for downstream applications such as Western blotting.

For more information on the FleX-IP immunoprecipitation kit, visit the company’s website via the link on the right.