High Quality Specific ChIP-validated Antibodies

20 Jan 2016
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

Chromatrap, a business unit of Porvair Sciences Ltd, has announced the launch of its own range of Chromatin Immunoprecipitation (ChIP)-validated antibodies.

Building on the company’s success in the solid-state ChIP market, which underpins modern epigenetic research, the addition of ChIP-validated antibodies further enhances the high performance application solutions that Chromatrap can provide researchers.

Chromatrap Product Development Manager, Dr Amy Beynon commented “The use of high quality and specific ChIP-validated antibodies is essential for the success of a ChIP assay. The antibody must recognise and bind to native protein that is bound to DNA. Antibodies from other applications do not always work well in ChIP. That is why our team has thoroughly investigated the top ten most referenced antibodies in recent epigenetic research papers.” She added “We are pleased to be able to offer these top quality validated antibodies to our customers alongside our expanding range of kits for ChIP assays of every type. This will give our customers confidence in their protocols and their results, safe in the knowledge that a ChIP-validated antibody was at the heart of their assay”.

Chromatrap currently offers a range of ChIP kits for qPCR or sequencing and has now launched the first nine antibodies in its Validated Antibody program which are H3, RNA pol II (S), RNA pol II (S5), H3K9me3, 5mC, AcH3, AcH4, AR and Sin3a.

Dr Beynon continued “It is our intention to expand the range as we continue to identify, source and validate antibodies for ChIP experimental use. We hope to have most of the major epigenetic marks validated over the next 12 months, please check our website for updates.”

Links

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.ChIP-SequencingImmunoprecipitationSecondary Antibodies