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High Performance ChIP Assays from Nanograms of Starting Material

Porvair Sciences Ltd
12 Oct 2017
Finn Price
Administrator / Office Personnel

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Chromatrap has demonstrated that its unique solid state patented technology allows for ChIP-seq assays to be routinely performed from as little as 500 ng of chromatin.

Traditionally availability of starting material has been a major problem for epigenetics researchers. The available techniques for tackling small samples which provide low yields of chromatin are usually long and complex, and involve pooling or the spiking of samples with chromatin from another source. Using Chromatrap® ChIP-seq assay kits, which give you an incredibly high signal-to-noise ratio, it is possible to start with samples as small as 500 ng and achieve high recovery of DNA for downstream applications such as next generation sequencing.

Do you use ChIP qPCR kits from Chromatrap? Write a review today for your chance to win an Amazon voucher worth $400 or an iPad Air®.

The Chromatrap® ChIP-Seq kit is a highly sensitive kit that can be used for both qPCR and sequencing. Utilizing patented solid-state technology, the Chromatrap® ChIP-Seq kit can deliver a streamlined ChIP-Seq protocol from tiny amounts of starting material and low chromatin concentrations. Specifically designed to work with broad chromatin concentrations, this new ChIP-Seq kit combines the dynamic range of Chromatrap® with the downstream analysis power of deep sequencing. This allows faster, more reproducible genome wide identification of transcription factor binding sites and specific DNA associated protein modifications.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ChIP-SequencingqPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.Chromatin

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High Performance ChIP Assays from Nanograms of Starting Material