High gene silencing efficiency in Jurkat cells

Available from Eppendorf is a new application note that describes how electroporation is used for the transfection of Jurkat cells with small interfering RNA (siRNA).

The efficient transfer of siRNA into cells is a prerequisite for a successful gene silencing approach. This protocol describes how to determine the best electroporation conditions (voltage and pulse duration) and the optimum concentration of siRNA. A high transfection rate and minimum cell damage result in a silencing efficiency of 85%.

For the electroporation experiments, the Eppendorf Multiporator^® and Eppendorf^® hypoosmolar electroporation buffer were used. The Multiporator applies electronically regulated microsecond pulses (high cell survival rate), in combination with the hypoosmolar buffer a high transfection yield is achieved.

To view the application note, please click here.