SelectScience
Join Free
Accelerating Science
NewsSeparations

High-Energy AXIMA-TOF<sup>2</sup>™ MALDI CID MS from Shimadzu Delivers Sensitivity, Flexibility

Shimadzu Corporation
27 Feb 2007

Product news

The AXIMA-TOF2™ high-performance MALDI TOF-TOF mass spectrometer from Shimadzu Scientific Instruments, Inc. (SSI) is making its PittCon debut at booth #1820 during this year’s show.

Representing the next generation in high-energy collision-induced dissociation (CID) MS/MS instrumentation, the AXIMA-TOF2 brings enhanced flexibility to life-science laboratories, significantly extending the type of analyses they can provide.

AXIMA-TOF2 includes Shimadzu Biotech’s intuitive software and XML data exporting as a standard features, so the unit delivers maximum results from minimum user input, making it ideal for use in open-access labs.

Beyond its use in proteomics, the AXIMA-TOF2 is adept at analyzing and processing polymers, oligonucleotides, SNPs, metabolites, lipids, carbohydrates and small molecules. Using high-energy CID to augment fragmentation of analytes, Shimadzu Biotech’s AXIMA-TOF2 mass spectrometer maximizes its curved field reflectron technology to deliver MS/MS data without the need for post-acceleration.

“The AXIMA-TOF2 makes best use of the proprietary reflectron technology to deliver real high-energy CID MS/MS fragments with optimal ion transmission,” noted Dr. Emmanuel Raptakis, product manager for Shimadzu Biotech. “This device is perfectly suited for a multitude of applications where sensitivity counts, ranging from LC-MALDI protein identification to solving challenging problems in de novo sequencing. In fact, high-energy CID MS/MS analysis of a wide variety of compound classes, including lipids, carbohydrates and even valued-added industrial polymers, is yielding important structural information previously out of reach with other, lower energy MS/MS instruments.”

The unit’s novel ion-gating technology affords industry-leading precursor ion isolation resolution, allowing the successful MS/MS analysis of challenging, complex mixtures. The curved field reflectron technology, under license from Johns Hopkins University, MD, US, has been updated to enhance the sensitivity and quality of high-energy CID MS/MS. Ion scattering is minimized by utilizing a totally gridless ion path and helium as the collision gas of choice.

The AXIMA-TOF2 key benefits include:

  • Highest energy collisions (20keV laboratory-frame collisions)
  • Outstanding sensitivity
  • Optimal precursor ion selection
  • Manual or fully automated operation for seamless analysis
  • High-resolution MS data in reflectron mode for accurate peptide mass fingerprinting
  • Proteomics analysis capabilities
  • LC-MALDI software for precise identification of off-line separated complex mixtures via automated MS/MS and integrated database searching

The AXIMA-TOF2 comes with multiple software features including:

  • Proteomics Suite for single sample manual acquisition or fully automated data dependent peptide mass fingerprinting and MS/MS for protein identification with integrated Mascot® searching.
  • LC MALDI Integrated Package provides total support for LC MALDI-based experiments with fully automated acquisition, including an intensity map of all sample spots across the target to assess the distribution of peptides and identify the position of the apex of chromatographic peaks, compilation of ‘candidate’ list, automatic MS/MS and subsequent database searching.
  • Biomarker Recognition identifies biomarker patterns and distribution compounds of interest in clinical samples. Data can be easily exported into alternative processing packages.
  • Functional Genomics offers the user excellent linear mode performance that lends itself to alternative applications such as oligonucleotide and SNP analysis and QC processes.
  • QC Applications, with Launchpad™ software, includes a module offering fully automated QC analysis of large numbers of samples, complete with a user-defined report indicating the presence or absence of the target compound, an estimate of purity and known contaminants, and adducts or truncated/extended analogues.

Links

Shimadzu CorporationCompany website

Tags

LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.SNPs TechnologySingle nucleotide polymorphisms (SNPs) are individual base variations in a DNA sequence. SNPs are used in research to study predispositions to disease and drug discovery. Products for studying single nucleotide polymorphisms include SNP arrays and detection systems to detect polymorphisms, SNP typing systems for genotyping DNA, and PCR to amplify specific SNPs.MALDI-TOF MSMALDI-TOF mass spectrometers (matrix-assisted laser desorption/ionization time-of-flight) are used for the mass analysis of large molecules, including proteins, DNA, polymers and other macromolecules due to its relatively soft ionization, reducing fragmentation whilst allowing fast data acquisition. Tandem mass analyzers such as TOF/TOF systems can be used to increase resolution and sensitivity of your analysis. Find the best MALDI-TOF products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

Related articles