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High Definition Data Directed Analysis: The Application of Quadrupole Ion Mobility Time-of-Flight Mass Spectrometry for Untargeted Proteomics Studies

Waters
9 Jul 2013
Sarah Thomas
Associate Editor

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This application note demonstrates the instrumental and application benefits for the identification of proteins and peptides in a new high definition data directed analysis (HD-DDA) mode, where ion mobility spectrometry is incorporated into a quadrupole time-of-flight mass spectrometer. HD-DDA uses a high duty cycle mode and enhanced decision making to provide a highly sensitive and selective experiment.

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LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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High Definition Data Directed Analysis: The Application of Quadrupole Ion Mobility Time-of-Flight Mass Spectrometry for Untargeted Proteomics Studies