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Have you read the new eBook on knock-out cell lines?

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Explore a compendium full of tips, tricks, and tools, and explore cutting-edge research on KO cell lines

26 Jun 2022
Rory Shadbolt
Publishing / Media

Editorial article

Gene knock-out (KO) technology and the generation of KO cell lines have been essential tools in biology for studying genes of unknown function. However, the advent of CRISPR-Cas9 KO technology is expanding the range of cell lines available for research far beyond previous offerings, rendering them cheaper and more reliable through advancements such as direct injection.

Knock-out cell lines eBook cover

Additionally, recent improvements to CRISPR, for example, in its range of companion CAS enzymes, have expanded the availability of DNA sequences that can be targeted. Companies like Abcam have been, and continue to be, at the forefront of new developments in KO cell line technology, and further improvements in the specificity of targeting and repairing genomic DNA look set to herald an exciting future for personalized medicine.

Download eBook for free

Download to explore in-depth descriptions, case studies, and resources on:

  • The importance of robust KO validation
  • Sourcing the right KO cell lines for your program
  • gRNAs for increased gene-editing success
  • Investigating signaling pathways such as PD-L1 and TRAIL

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene EditingGene editing is the precise alteration to an organism's DNA, with CRISPR being the most well-known technique. It is used in a wide range of applications, including the development of disease models and gene therapy, and crop improvement. Browse our peer-reviewed product directory to find the best gene editing solutions, compare products, check reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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