Harnessing knockout validation for reproducible research

Join us on Thursday, 9 July, to learn how Abcam applies KO validation at a large scale and the lessons learned from implementing this initiative

24 Jun 2020
Georgina Wynne Hughes
Editorial Assistant

Expert insights

Dr. Hanna Dreja, Principal Scientist, In-house Manufacturing, Abcam
Dr. Hanna Dreja, Principal Scientist, In-house Manufacturing, Abcam

The lack of target specificity seen in some antibodies has contributed to the life science ‘reproducibility crisis’. Knockout (KO) validation is one of the most accepted and trusted processes to confirm the specificity of an antibody.

In this upcoming webinar, hear from Dr. Hanna Dreja, Principal Scientist at Abcam, to discover and learn how Abcam applies KO validation at a large scale and the lessons learned from implementing this initiative. From the off-target effects exhibited by some of the top-cited antibodies to the average time to generate specific KO cell lines, you will find out the caveats of KO validation and how you can apply this technology to your research, to achieve reliable results, faster.

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Key learning objectives

  • Understand the benefits of working with knockout cell lines both as a technical validation tool and to unlock key biological questions
  • Gain expert insight on how knockout cell lines are being applied from basic research and target discovery to pharmacogenomics
  • Learn how to overcome the common challenges of working with knockout cell lines

Who should attend?

This webinar will provide insights for researchers in:

  • Cell biology and disease
  • Validation and quality control
  • Drug discovery and assay development
  • Research projects involving antibody validation and KO cell lines

Certificate of attendance

All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes.

Register to watch the full webinar here>>

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Quality ControlQuality control is needed in all production processes. Quality control is a monitoring procedure or set of procedures that are put in place to ensure that a manufactured product adheres to a defined set of quality criteria.