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GE Healthcare Launches Mag Sepharose™ Beads

Cytiva
22 Jul 2009
Sarah Sarah
Marketing / Sales

Product news

GE Healthcare has launched Mag Sepharose™ magnetic beads for the efficient capture and selective enrichment of proteins and peptides. The visible, dense magnetic beads are easy to use, with simple, flexible protocols and elution conditions optimized for both electrophoresis and MS analysis.

The Mag Sepharose beads are available within the following formats:

• TiO2 Mag Sepharose for the enrichment of phosphopeptides from complex samples
• Protein A and Protein G Mag Sepharose affinity chromatography media for the capture of target proteins by immunoprecipitation
• NHS Mag Sepharose for covalent coupling of any protein containing free amine groups, used for various types of pull-down experiments

The magnetic beads are well suited for small-scale experiments. The high density of the beads allows rapid capture by magnetic devices, while the visibility of the beads ensures reliable collection of all target protein. This allows concentration of low abundance target proteins in samples from milliliter down to microliter scale. The optimized capacity for enrichment or immunoprecipitation means that only small amounts of capturing antibody are required.

The new Mag Sepharose beads from GE Healthcare are available in two pack sizes: 1 x 500 µl which is sufficient for 10 samples; and 4 x 500 µl which is sufficient for 40 samples. When used in conjunction with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. The rack consists of an anodized aluminum housing with a detachable plastic bar containing six neodymium magnets. When the tubes are placed in the rack, the beads are attracted to the magnet facilitating easy removal of the supernatant whilst the beads remain in the tube.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.

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GE Healthcare Launches Mag Sepharose™ Beads