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GE Healthcare Launches HiTrap™ Con A 4B Prepacked Columns

Cytiva
15 Jun 2009
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GE Healthcare announces the launch of HiTrap™ Con A 4B, ready-to-use columns prepacked with Con A Sepharose™ 4B, a chromatography medium for separation and purification of glycoproteins, polysaccharides and glycolipids.

The HiTrap columns are made of biocompatible polypropylene and are available in 1 ml and 5 ml formats, providing a convenient and reproducible solution for separations. These columns can be operated with a syringe, a peristaltic pump, or a liquid chromatography system such as ÄKTAdesign™

Con A Sepharose 4B is an affinity chromatography medium with a lectin, concanavalin A (Con A), coupled to Sepharose 4B. Immobilized lectins are invaluable tools for isolating and separating glycoproteins, polysaccharides and glycolipids, subcellular particles and cells, and for purifying detergent-solubilized cell membrane components. They are also useful for assessing changes in levels or composition of surface glycoproteins during cell development and in malignant or virally transformed variants.

Con A Sepharose 4B chromatography medium is also available in 5 ml and 100 ml bulk packs.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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GE Healthcare Launches HiTrap™ Con A 4B Prepacked Columns