SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Full-Length Proteins from Formalin-Fixed Tissue

QIAGEN
23 Oct 2006

Product news

As the standard method for histological tissue preparation, millions of Formalin-Fixed Paraffin-Embedded (FFPE) samples are classified and archived each year.

Now, the Qproteome FFPE Tissue Kit opens this vast, highly valuable resource up to proteomic and protein expression analysis by enabling efficient extraction of full-length proteins suitable for western blotting and protein arrays.

Because formalin fixation creates chemical crosslinks, stabilizing samples for histological analysis but rendering any subsequent protein analysis (e.g., by western blotting) impossible, analysis of FFPE tissue has been limited to histochemical techniques. The Qproteome FFPE Tissue Kit uses innovative proprietary chemistry to reverse the crosslinking process and deliver full-length proteins ready for analysis by western blotting and protein array, with subsequent immunodetection. For the very first time, proteins or pathways of interest can be investigated in archived FFPE tissues.

The Qproteome FFPE Tissue Kit offers a unique tool for analysis of samples whose pathological status has been determined, but whose precise protein makeup has until now remained inaccessible. Such samples — for example, taken over a course of treatment or from different groups of probands — offer a rich source of information for comparative proteomics and biomarker studies.

Links

QIAGENCompany website

Tags

Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

Related articles

Full-Length Proteins from Formalin-Fixed Tissue