SelectScience
Join Free
Accelerating Science
NewsLife Sciences

Free Webinar: New Bio-Inert Hardware and LC bioZen Column Supports Biosimilar and Protein Characterization Complexity

SelectScience

Find out how you can save you time, avoid any issues with reproducibility, and improve both the robustness and quality of methods and data

16 May 2018
Holly McHugh
Administrator / Office Personnel

Expert insights

When performing analytical LC method development for proteins, priming of columns before analysis can be labor and time intensive, and often result in reproducibility and carryover concerns. A novel bio-inert column hardware has been designed to mitigate these problems and improve the quality of results by advancing levels of characterization.

As the complexity of biotherapeutics and biosimilars continues to increase, so too have the levels of characterization needed. To support this shift, Phenomenex has designed an LC column portfolio packed in bio-inert hardware, based on two high-efficiency, yet complementary particle platforms and a flexible collection of orthogonal surface chemistries. This new HPLC/UHPLC column portfolio for biologics, aptly named bioZen, focuses on providing improved reproducibility, resolution and performance across major protein characterization techniques.

In this educational webinar, we will introduce the new bio-inert hardware and discuss how it can help save you time, avoid any issues with reproducibility, and improve both the robustness and quality of methods and data.

Register now

We will explore:

  • The impact of hardware on sample recovery and priming
  • New HPLC and UHPLC portfolio for analysis of mAbs, ADCs and impurities
  • High molecular aggregate characterization and improved recovery with unique, thermally modified, fully porous SEC columns
  • Improvements in peptide mapping using orthogonal selectivities and particle platforms
  • Top- and middle- down approaches for antibody characterization including average DAR by reversed phase
  • A novel core-shell HILIC stationary phase that offers differentiated released glycan retention profile

Who should attend:

  • Anyone characterizing mAbs, ADCs or biotherapeutics by HPLC/UHPLC
  • Anyone new to the characterization of proteins by SEC, RP, HILIC or IEX
  • Anyone who has a bio-inert UHPLC system
  • Anyone interested in novel HILIC chemistries for N-linked glycan analysis

You can attend the live webinar on Wednesday, May 23, at:

  • 16:00 BST
  • 17:00 CEST
  • 11:00 EDT
  • 08:00 PDT

Don't worry if you can't make the live event, register to watch on demand at a time that suits you

Links

SelectScienceCompany website

Tags

ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. BiotherapeuticsBiotherapeutics are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or in <i>in vivo</i> diagnostics. The most well-known example of a biotherapeutic product, and the first to be approved for therapeutic use, was recombinant human insulin.Protein StructureProtein structure refers to the three-dimensional arrangement of amino acids in proteins, determining their function. Understanding protein structure is key in drug discovery, enzymology, and molecular biology. Explore protein structure analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

Related articles