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Free Webinar: Multiplexed Illumina Sequencing: Eliminating False Positives by Mitigating Sample Misalignment

SelectScience

Learn how to improve your NGS data reliability

2 Apr 2018
Frankie MacDonald
Administrator / Office Personnel

Expert insights

Brad Hehli, NGS scientist of Bioo Scientific, Perkin Elmer, will deliver this fascinating insight into dual-indexing strategies in NGS

Genomic science has evolved tremendously over the last few decades. Whilst the human genome project took 15 years and billions of dollars to complete, we can now sequence multiple genomes in one day for a fraction of the cost. With the launch of next generation sequencing technologies in the early 2000s, DNA sequencing has become a much more accessible tool that has accelerated disease research worldwide.

Advances in NGS technology now enable us to sequence multiple samples in simultaneous runs, by introducing unique reference barcodes during the library preparation process. However index mis-assignment, also known as index-hopping, is commonly encountered during multiplexing and can lead to the introduction of false-negatives in the data analysis.

In this upcoming educational webinar you will hear from NGS expert Brad Hehli, who will discuss methods to overcome this shortfall of Illumina sample multiplexing by dual-indexing strategies. Brad Hehli is an NGS scientist of Bioo Scientific, a Perkin Elmer company, with an interest in the metagenomic applications of next generation sequencing.

Register now

Register for this webinar to learn about:

  • Issues related to potential sequencing artefacts
  • How to improve the accuracy of your NGS data
  • New strategies to avoid sample misassignment

Attend the live webinar on, Wednesday April 11 at:

  • 16:00 BST
  • 17:00 CEST
  • 11:00 EDT
  • 08:00 PDT

If you can’t attend the live event, register to watch the webinar on-demand at a time that suits you

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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.SequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.Genome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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