SelectScience
Join Free
Accelerating Science
NewsLife Sciences

FREE WEBINAR: Learn how to capture antibody internalization in real time using live-cell analysis

SelectScience

Sign up now for this free webinar where you’ll learn an alternative to an otherwise laborious and time-consuming protocol

5 Mar 2018
Anita Ramanathan
Administrator / Office Personnel

Expert insights

Antibody drug conjugates (ADCs), used widely as anti-cancer, anti-viral and anti-inflammatory therapeutics comprise of monoclonal antibodies (mAbs) attached to biologically active drugs via chemical linkers. While the mAbs recognize tumors (or a desired type of cell) with high specificity, the drugs impart cytotoxic properties to kill the cells. An important property of antibodies is the extent, location and rate of internalization into the targeted cells.   Image courtesy of Essen Bioscience.

Current methods to directly quantify antibody internalization are laborious and time-consuming; they use a fluorescent tag where signals of the internalized antibodies need to be isolated from the ‘un-internalized’ ones on the outside of cells. Plus, they do not test multiple antibodies. Almost all methods (for example, flow cytometry, high-content imaging) provide only end-point assays, thereby requiring multiple experiments to follow internalization over time.

An upcoming webinar on SelectScience will provide you with an integrated assay solution to visualize antibody internalization in real time. Our presenter, Dr. Nicola Bevan, Principle Scientist at Essen BioScience and an experienced pharmacologist, will explain how you can use the IncuCyte FabFluor-pH reagent to provide a turn-key approach to antibody labeling and obtain insights from live-cell imaging.

Register now

WHAT: Real-time antibody internalization assays using live-cell analysis
WHEN: Wednesday, March 14, 2018
Time: 16:00 GMT / 17:00 CET / 11:00 EST / 08:00 PST
Reserve a space here >>

In this webinar you will learn:

  • How to visualize and analyze antibody internalization in real time
  • How to use the IncuCyte FabFluorpH reagent to provide a turn-key approach to antibody labeling
  • Examples of how the assay may be used for characterization of early-stage test antibodies and candidates in a biologics discovery paradigm

Many scientists from around the world have already registered for this free event. Don’t miss out. Reserve a space now!

If you can’t make it to the live event, no problem, you can always sign up to receive an on-demand link here.

Links

SelectScienceCompany website

Tags

AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.Antibody Drug ConjugatesEngineered AntibodiesEngineered antibodies are modified to enhance specificity, stability, and functionality for therapeutic and diagnostic applications. These antibodies are central to immunotherapy and biomarker detection. Compare engineered antibody solutions in our directory to find top products and reviews.

Related articles