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Free Webinar: Enhancing Reversed Phase Separations for Better Characterization of Protein Therapeutics

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Dr. Davy Guillarme, University of Geneva, presents a high throughput, high resolution RPLC method for efficient separations of proteins

16 Feb 2018
Sarah Thomas
Associate Editor

Expert insights

Do you find it challenging to achieve efficient separations of proteins using reversed-phase liquid chromatography (RPLC), due to their size and complexity? Well, there is good news for anyone carrying out protein RPLC as our forthcoming expert webinar with leading analytical chemist Dr. Davy Guillarme, of the University of Geneva, and Waters R&D scientist Jennifer Nguyen will show.


New RPLC column technology has been developed that marries the benefits of a phenyl-based surface chemistry with the kinetic advantages of solid-core particles. From the capabilities afforded by these developments, higher-throughput, higher-resolution separations have been achieved with lower temperatures and lower levels of ion pairing. It has therefore been possible to minimize on-column degradation, facilitate MS-based peak identification, and thereby obtain higher fidelity data for detailing the heterogeneity of protein therapeutics, as can be highlighted with the development and application of new methods for the characterization of monoclonal antibodies (mAbs) and antibody drug conjugates (ADCs).


This webinar will explore:

  • The impact of column technology design on protein RPLC
  • Method development parameters
  • Strategies to improve throughput and resolution for protein characterization
  • All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes.

Register now

This webinar takes place on Tuesday, March 6, 2018 at:

  • 16:00 GMT
  • 17:00 CET
  • 08:00 PST
  • 11:00 EST

If you can't attend the webinar at this time, you can still register to catch up at a later date that suits you.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Monoclonal AntibodiesMonoclonal antibodies (mAbs) are lab-made molecules that can bind to specific antigens on cells, making them highly effective in treating cancers, autoimmune diseases, and infections. These targeted therapies are transforming the treatment landscape for many conditions. Browse our peer-reviewed product directory to find monoclonal antibodies for research and clinical applications; compare products, check reviews, and get pricing directly from manufacturers.Protein SeparationAntibody Drug ConjugatesBiotherapeuticsBiotherapeutics are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or in <i>in vivo</i> diagnostics. The most well-known example of a biotherapeutic product, and the first to be approved for therapeutic use, was recombinant human insulin.Engineered AntibodiesEngineered antibodies are modified to enhance specificity, stability, and functionality for therapeutic and diagnostic applications. These antibodies are central to immunotherapy and biomarker detection. Compare engineered antibody solutions in our directory to find top products and reviews.Reversed-Phase LC

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Free Webinar: Enhancing Reversed Phase Separations for Better Characterization of Protein Therapeutics