SelectScience
Join Free
Accelerating Science
NewsSeparations

Free Webinar - Considerations for Selecting the Optimal Stationary Phases for Proteomic Trap-and-Elute NanoLC-MS

SelectScience

Learn how the trap-and-elute NanoLC-MS system enables high-throughput, highly sensitive proteomic separation that overcomes small volume limitations of nanocolumns

16 Jan 2018
Frankie MacDonald
Administrator / Office Personnel

Expert insights

Liquid chromatography-mass spectrometry (LC-MS) is a highly efficient separation platform for identifying compounds in complex mixtures by combining physical separation with mass analysis . The emergence of Nanoscale LC technology allows for greater sensitivity to be achieved, and enables increased spectrometric resolution when coupled with mass spectrometry. Whilst this approach is particularly useful when sample availability or target peptide concentration is low, it is not suitable for large-volume sample separations.

The trap-and-elute method for nanoflow LC-MS, however, can overcome the limitations of small volume nanocolumns by allowing for relatively large volumes to be injected without compromising sensitivity. Further benefits of the nano LC-MS configuration can include the prevention of column clogging and build-up of impurities, as well as flexibility with the nanocolumn stationary phase.

Join us for this free webinar, in which Moon Chul Jung, Ph.D., of Waters Corporation, discusses the advantages of the trap-and-elute nanoLC-MS system and considers common configurations for optimal proteomic separation using the nanoflow system.

Registration for this live event on Wednesday, January 24, is now open. Don’t worry if you cannot attend, register here and you can watch this webinar on demand at a time that is more convenient for you.

In this webinar you will learn about:

  • Common trap-and-elute system configurations and benefits
  • Factors influencing the efficiency of trap-and-elute nanoLC methods
  • Understanding and utilizing retentivity of stationary phases

This webinar will go live, Wednesday January 24, at:

  • 11.00 EST
  • 16:00 GMT
  • 17:00 CET
  • 08:00 PST

If you can't attend the live event, simply register to watch the webinar on demand at a time that suits you.

Links

SelectScienceCompany website

Tags

NanoLCNano LC is a nano scale liquid chromatography technique typically used for proteomic applications. The nano refers to the very low flow rate of the mobile phase in the system. The advantage of nanoLC is its high sensitivity. Often the system is integrated with a mass spectrometer in nano LC-MS or nano LC-MS/MS. When using nanoLC equipment consider column choice, software and accurate and precise flow rate control.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Protein SeparationChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.

Related articles