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Free Webinar - Comparative Analysis of FFPE-Derived RNA: A Novel Relationship Between RNA Quality and Sequencing Results

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Learn how to optimize FFPE sample preparations to enable successful downstream assays

12 Dec 2017
Lui Terry
Administrator / Office Personnel

Expert insights

RNA derived from formalin fixed, paraffin embedded (FFPE) tissues are invaluable for gene expression analysis of a myriad of disease states. However, the labile nature of RNA and the ubiquitous presence of RNase makes RNA difficult to work with throughout the FFPE sample preparation process and can severely affect the functionality of RNA downstream.

While the advent of robust next-generation sequencing (NGS) technologies has enabled low-quality and low-input RNA for RNAseq workflows, the reliability and accuracy of RNAseq data is still dependent on the extent of template RNA quality and amount. Interrogating the FFPE-derived RNA quality is a critical and yet overlooked step towards achieving optimal NGS results.

Join us for this free webinar, in which Dr. Jung Doh, senior applications scientist at Beckman Coulter Life Sciences, will discuss how the tissue fixation process affects RNA quality and influences downstream experiments such as NGS. The webinar will then provide solutions to overcome some of the challenges in RNA extraction from FPPE extractions and be followed by a Q&A session.

Registration for this live event on Friday, December 18, is now open. Don’t worry if you cannot attend, register here and you can watch the webinar on demand at a time that is more convenient for you.

In this webinar you will learn about:

  • The differences between high, low and medium quality RNA
  • The types of RNA quality metrics and methods of analysis used today
  • Why it is difficult to isolate good-quality RNA from FFPE tissues
  • The importance and effects of RNA quality and extraction methods in RNAseq workflows and outcomes
  • Countermeasures to optimize FFPE sample preparations to enable successful downstream assays

This webinar will go live Friday, December 18, 2017, at:

  • 11.00 EST
  • 16:00 GMT
  • 17:00 CET
  • 08:00 PST

If you can’t attend the live event, please register to watch the webinar on demand at a time that suits you.

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DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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