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Free SelectScience Webinar: Tips and Tricks for LC-MS

SelectScience

Register your place for this exciting event and Q&A session

13 Sept 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Expert insights

Optimization of your LC-MS system and workflow is critical to ensure minimal instrument downtime and troubleshooting. While sample preparation is often prioritized when looking to streamline LC-MS workflow, the operation of your instrument is just as important to extend its life and improve data quality. LC-MS systems can be expensive and time-consuming to maintain, but are able to sensitively analyze complex samples and detect very similar compounds. Therefore, it is crucial that your LC-MS is running as efficiently as possible to ensure the benefits of LC-MS analysis are maximized.

In this webinar, Hans Griesinger, Analytical R&D Engineer, Merck KGaA, will discuss tips and tricks to optimize the coupling of liquid chromatography with mass spectrometry.

By watching this webinar, you will learn how to:

  • Optimize your LC-MS system and minimize instrument downtime
  • Enhance mobile phase quality, buffers and solvent storage
  • Clean your LC-MS equipment, including column bleeding and washing
  • Analyze complex samples, as demonstrated in a series of case studies

The Q&A session during the webinar will give you the opportunity to ask any questions you may have for Hans.

The webinar will take place at 07:00 PDT / 10:00 EDT / 15:00 BST / 16:00 CEST, September 24, 2015, and will be available on-demand if you are unable to attend the live event.

Register today for your free place.

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High purity solvents for LC-MS and trace analysis with UV and fluorescence detection: 0.2 µm stainless steel filtered – perfect for use in UPLC and UHPLC. Very high UV-transmittance. Excellent baseline stability in gradient elution. Very low total ionic current (TIC) in LC-MS. Low level of ionic background and ion suppression. High ionization efficiency in all ionization methods (electrospray ionization/atmospheric pressure chemical ionization (ESI/APCI) positive and negative mode). Applications: Protein profiling. Pesticide Analysis. Polycyclic aromatic hydrocarbons (PAHs). Proteomics LC-MS. LC-MS routine analysis. Lichrosolv® hypergrade solvents enable analysis in the low ppb trace range and can be used for both the isocratic separation of 6 PAHs according to the German DIN method and the gradient separation of 16 PAHs according to the methods EPA 610 (analysis of drinking water) and EPA 550 + benzo(e) pyrene + perylene (analysis of waste water).

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High Purity Solvents for headspace GC and LC-MS

Merck KGaA, Darmstadt, Germany

SupraSolv® high-purity solvents for headspace GC SupraSolv® solvents for headspace Gas Chromatography (GC) are optimized for the analysis of residual solvents in drug substances, excipients, and drug products according to the International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH), the European Pharmacopoeia (Ph Eur) and the United States Pharmacopoeia (USP). Thanks to their superior baseline clarity and extremely low solvent residues, they eliminate any interference with your findings. All residual solvent classes defined in the ICH guideline are included in the specification, providing you with a precise purity window for your application. Specified according to ICH, Ph Eur and USP Reliable and reproducible results Batch-to-batch consistency LiChrosolv® hypergrade solvents for LC-MS LC-MS, the combination of classical liquid chromatography (LC) with mass spectrometry (MS), is essential in virtually every field of chemical analysis. LiChrosolv® hypergrade solvents are specifically produced and accurately tested for LC-MS suitability, and meet all the requirements of modern LC-MS ionization methods (ESI/APCI positive and negative mode) for best quantitative in triple and quadrupole performance. Due to their low level of ionic background and low ion suppression, they excel in reproducibility and ionization efficiency. The extensive range of returnable stainless steel barrels and withdrawal systems are designed for safe and effortless use to avoid the risk of contamination. Proven LC-MS suitability Highest batch-to-batch consistency High ionization efficiency Excellent baseline clarity

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Chromolith® High Resolution HPLC Columns

Merck KGaA, Darmstadt, Germany

Chromolith® HighResolution: High resolution separations without ultra high pressure. The revolutionary monolithic Chromolith® HighResolution columns are made from a single rod of high-purity monolithic silica, rather than from particles. Their unique bimodal pore structure allows the chromatographic performance to be improved significantly. Speed of analysis (separations up to 9 times faster if required) Separations two times faster at half the column back-pressure compared to μm columns Higher sample throughput Improved HPLC system security Significantly increased column lifetime Reduced maintenance on HPLC pump and injector seals Reduced need for sample preparation as columns are very resistant to blocking (even with biological samples) Column length no longer pressure limited Very high separation efficiency by column coupling

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Solvent MonitoringLow Bleed ColumnLC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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