Fourth New Fluorescence Application of Dyversity Makes Image Analyser Best in Class for 1D and 2D Protein Gel Imaging

Syngene, a world-leading manufacturer of image analysis solutions, is pleased to announce, Dyversity, its new multi-functional imager can detect nanogram quantities of protein stained with Invitrogen’s Pro-Q® Diamond in seconds, providing an excellent method for proteomic researchers who want to rapidly image 1D and 2D protein gels.

Using a Dyversity system fitted with a Cy dye lighting module, dual wavelength transilluminator, UV and Cy3 dye emission filters, Syngene’s technical experts imaged 1D acrylamide gels containing 1000-0.1ng Pro-Q Diamond stained phosphoprotein molecular weight standard, PeppermintStick™ (Invitrogen). The gel images were captured using two different settings: Cy3 excitation with a Cy3 emission filter and medium-wave UV excitation with a UV emission filter.

Syngene’s application specialists found both imaging conditions of Dyversity produced comparable results with identical exposure times, detecting as little as 5ng of Pro-Q Diamond stained protein in less than 3 seconds. This result coupled with the fact Dyversity can also rapidly detect nanogram amounts of other fluorescent proteomics stains such as Deep Purple™, SYPRO® Ruby and Flamingo™, as well as traditional visible stains including Coomassie® Blue, means the Dyversity system is an excellent, cost-effective alternative to laser scanning for imaging a variety of protein stained gels.

Laura Sullivan, Syngene’s Divisional Manager explained: “We have designed Dyversity 6 to include a high resolution 6.3 mega pixel CCD camera so that unlike a laser scanner the system can simultaneously excite at the optimum fluorescent or visible light excitation peak across an entire gel, making image acquisition a task that takes seconds rather than minutes. Additionally, since Syngene has determined the optimal imaging conditions for detecting small quantities of protein stained with Pro-Q Diamond and a range of many other common protein dyes, Dyversity is fast becoming the perfect system for gel based proteomics research.”