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FastLane Kits - from sample direct to result

QIAGEN
23 Jan 2007

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FastLane Kits accelerate and streamline real-time RT-PCR analysis of cultured cells. By eliminating the need for RNA purification, the kits allow you to carry out real-time RT-PCR directly from cell lysates. The kits are ideal for experiments requiring rapid, high-throughput gene expression analysis, such as validation of siRNA-mediated gene knockdown.

Only 12 minutes are required to prepare cell lysate which can be used directly in reverse transcription or in real-time, one-step RT-PCR. To ensure reliable gene expression analysis in subsequent real-time RT-PCR, RNA is immediately stabilized upon cell lysis and genomic DNA is effectively eliminated using novel gDNA Wipeout Buffer.

The comprehensive range of FastLane Kits provides high-speed solutions for all types of real-time RT-PCR. Kits are available for one-step RT-PCR (for cell-cultures of up to 384-wells) and two-step RT-PCR (for cell-cultures of up to 96-wells). Quantification is achieved using sequence-specific probes (in single or multiplex reactions) or SYBR® Green I dye.

FastLane Kits are intended for research use. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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FastLane Kits - from sample direct to result