Fast Protein Purification LC at BIOTECHNICA 2015

22 Sept 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

KNAUER is offering free tickets for BIOTECHNICA in Hannover, Germany, and will be presenting the AZURA® Bio LC systems at booth A35, hall 9, ideal for fast protein purification.

Adapting to any biochromatography purification challenge is very easy with the modular AZURA® Bio LC platform. These LC systems are reported to be scalable regarding the purified amount of protein and the desired level of automation. The buffer supply can be chosen for maximum flow rates from 10 ml/min up to 1000 ml/min, including gradient options or a buffer selection option. Column switching, peak parking, different fraction collection choices and detection principles are also available, so that users are able to easily meet changing requirements at any time.

Typical applications include affinity chromatography, size exclusion chromatography, ion exchange chromatography, hydrophobic interaction chromatography, as well as mixed mode chromatography. The systems are combinable with all common LC columns in the market.

Unique in this field is the possibility of the purification software PurityChrom® Bio, to develop and work with methods based on volume, column volume or time. The software offers practical safety features protecting the valuable product, e.g. a “hold function” that can be invoked during a run or a solvent level monitoring function to prevent unintended running out of buffer.

A particularly clever function is the waste management function, that collects waste-per-run, which is very useful during method development or when sequentially handling different samples. In case anything goes wrong with the separation, the user has the option to get the sample from the “dedicated” waste vial for this sample. This will then require an additional fractionation valve or fraction collector to “sub-divide” the waste.

The AZURA Bio LC systems can also be controlled with other chromatography data systems with a purification function, such as time-based ClarityChrom® and OpenLAB® EZChrom Edition packages.

Summarized, KNAUER is presenting a very open platform for FPLC applications, leaving much choice to the user. Learn more about KNAUER's open FPLC platform, and get your free ticket today!

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AutomationAutomation in laboratories and manufacturing processes enhances efficiency, precision, and scalability by reducing the need for manual intervention. It plays a critical role in improving productivity, minimizing human error, and accelerating workflows in fields like diagnostics, drug development, and industrial testing. Automation technologies include robotic systems, automated liquid handlers, and process control systems that streamline complex tasks and ensure consistent, reproducible results. Explore our peer-reviewed product directory to discover the best automation solutions, compare options, read user reviews, and get prices directly from manufacturers.Method DevelopmentMethod development is the process of creating and optimizing experimental techniques and protocols to achieve reliable, reproducible results. This is essential in various fields, including pharmaceuticals, environmental science, and diagnostics. Browse our peer-reviewed product directory to find the best method development solutions, compare products, check reviews, and get pricing directly from manufacturers.Biotechnica