Fast Liquid Transfer Down to Picoliter Levels with Tecan’s D300e

30 Jun 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

Tecan has introduced the D300e Digital Dispenser for applications that need faster, reliable dispensing down to picoliter volumes. This innovative, simple-to-use device is ideal for the creation of assay plates, and can cut set-up times from hours or days to just minutes. Users can choose between dispensing aqueous solutions in combination with a surfactant – for the investigation of proteins, antibodies, enzymes and nucleic acids – and DMSO, for small molecule studies, offering greater flexibility for life sciences workflows.

The system’s sophisticated yet simple software guides you through every operation, minimizing training times and problem-solving call-outs, and providing straightforward set-up of enzyme profiles, dose-response curves and synergy studies. Even the most complex experimental plate layouts – such as drug-drug interaction studies – can be quickly and easily generated, with optional randomization to reduce the impact of edge effects and increase data integrity. The D300e is compatible with a wide range of microplate formats from 12 to 1,536 wells, including deep-well plates and, for maximum flexibility, can even accommodate SBS-format tube racks.

i-control™ Microplate Reader Software

Tecan

The i-control microplate reader software is a user-friendly interface for control of Tecan’s Infinite® series of microplate readers, enabling straightforward definition of workflows for various applications.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Assay AssemblyAssay Assembly is technique used in drug discovery to develop assays to test the cytotoxicity, genotoxicity, or other activities of a compound on a cell. Assay assembly requires chip assembly, a delivery system and a detection and analysis method. Beneficial features of assay kits or automated systems include high-throughput, high speed and sensitivity and low signal to noise ratio.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.Antibody ArraysAssay DevelopmentThe process of proving an assay to be sensitive with respect to the target is known as assay development. The assay should be able to characterize novel compounds and measure the potency of these compounds against a validated biological target.DMSO
Fast Liquid Transfer Down to Picoliter Levels with Tecan’s D300e