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Fast and Efficient Preparation of Next Generation Sequencing Libraries

Merck
14 Aug 2012
Tesni Perry
Administrator / Office Personnel

Product news

EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, today launched PureGenome™ kits and reagents for rapid and efficient Next Generation Sequencing (NGS) sample preparation. With these reagent sets, library preparation has been streamlined to two steps in under two hours, followed by a short enrichment step, thus alleviating a typical bottleneck in the sequencing process.

PureGenome™ library preparation is a simple, two-step process followed by amplification using EMD Millipore's ultra-high fidelity KOD Hot Start DNA Polymerase Mastermix. This unique polymerase amplifies DNA with high processivity in highly TA- or GC-rich regions. The combined efficiency of library construction and accuracy of amplification enables maximum library yields from lower input DNA with minimal bias.

Sequencing data from libraries prepared using the PureGenome™ NGS library preparation reagents are of the highest quality with respect to Phred scoring (Q20>85%), minimal bias in GC coverage and higher ratios of matched:unmatched reads. Ultimately, better sequencing data translates into more meaningful biological results, especially in applications for which sensitivity and specificity are crucial, such as gene expression analysis or single-nucleotide polymorphism studies. The PureGenome™ NGS library preparation reagents are validated for Illumina® platform-compatible NGS libraries; however, end users have the flexibility to optimize for other platforms.

"To date the cost and sample processing rate has been a bottleneck in NGS, and the choice of library preparation products has been somewhat limited," notes John Sweeney, Executive Vice President, EMD Millipore Bioscience Division. "PureGenome™ NGS library preparation reagents require less hands-on time and less overall processing time compared to similar platforms. In addition, the quality of sequencing data surpasses that of similar platforms with greater cost efficiency."

Supporting kits in the PureGenome™ workflow include the PureGenome™ Tissue DNA Extraction Kit, specifically validated for NGS library preparation from solid tissue, biological fluids and cell cultures, and the PureGenome™ On-Spot Tissue DNA Kit, which is used for extraction of DNA from targeted areas on tissue sections and enables analysis from minute samples. The PureGenome™ NGS Library Validator Kit enables highly accurate and cost-effective qPCR quantification of Illumina® platform-compatible NGS libraries.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Labware and Lab SuppliesLabware and lab supplies include various forms of glassware, plasticware, reagents and personal protective equipment (PPE) and are used across all scientific laboratories, enabling daily tasks to be performed. Explore a range of flasks and beakers, petri dishes, carboys and storage bottles. Labware such as condensers, desiccators and burettes are also available for more specific tasks. Find the best labware and lab supplies in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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