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Exploring single-cell biophysics with a simple, robust microfluidics system

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Join this expert webinar for a review of the power of microfluidics for dynamic time-lapse experiments

6 May 2020
Georgina Wynne Hughes
Editorial Assistant

Expert insights

Sunil Desai, Research Technology Specialist at MilliporeSigma and Dr. Enrique Rojas, Assistant Professor of Biology at New York University
Sunil Desai, Research Technology Specialist at MilliporeSigma and Dr. Enrique Rojas, Assistant Professor of Biology at New York University

Live-cell analysis monitors rapidly changing living processes. Scientists recognize the need for observing cellular changes over extended periods of time to truly understand biological systems. A major limitation with traditional static cell culture methodologies is the lack of a highly controlled, in vivo-like dynamic environment.

In this exclusive webinar, Dr. Enrique Rojas, Assistant Professor of Biology at New York University, and Sunil Desai, Research Technology Specialist at MilliporeSigma, will review the power of microfluidics for dynamic time-lapse experiments. Beyond this, a perfusion-based control system integrating imaging capabilities will be introduced, which permits optimal cell health over long time periods, and provides the ability to precisely manipulate environmental parameters and measure cellular responses in real time.

Rojas will describe his application subjecting bacteria to rapid oscillatory osmotic shocks for studying the biomechanical basis of cell growth and division. In gram-negative strains, Rojas demonstrates the outer membrane’s significance for sustaining mechanical perturbations and growing in the presence of antibiotics. Additionally, Rojas discusses the ecology of bacteria-bacteriophage interactions and the genetic basis of cell growth.

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Key learning objectives:

  • The use of microfluidics for time-lapse microscopy.
  • How to monitor cells (single-cell level) in real time, while delivering precise and rapid changes to culture parameters, including temperature, media and gas.
  • Understand perfusion cell culture systems that provide optimal representation of natural physiological conditions.
  • How microfluidic systems integrated with inverted microscopy allow continuous, high magnification observation of live cells, while capturing their reactions to environmental perturbations in real time.

Who should attend?

  • Researchers who use live-cell imaging
  • Researchers who study host-pathogen interactions, protein translocation, hypoxia, cell migration, autophagy, 3D cell culture and primary neuronal cultures.
  • Researchers conducting time-lapse microscopy
  • Researchers utilizing bacterial, mammalian or yeast systems

The live webinar and Q&A session will take place on Thursday, May 21, at:

  • 18:00 BST
  • 19:00 CEST
  • 10:00 PDT
  • 13:00 EDT

Register to watch the full webinar>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.In Vivo Imaging Systems<i>In vivo</i> imaging systems, including pre-clinical imaging systems and medical imaging systems are used to non-invasively visualize and capture images of live animals and plants. Monitor the natural processes or diseases of your subjects using small-animal pre-clinical imaging systems, including single photon positron emission tomography (SPECT), positron emission tomography (PET), computed tomography (micro-CT), magnetic resonance imaging (MRI), X-ray radiography, ultrasound, fluorescence and bioluminescence imagers. Multimodal systems and software solutions are also available for correlative analysis of organ, tissue, cell, or molecular-level processes. Find the best in vivo imaging products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.MicrofluidicsMicrofluidics is the science of manipulating small volumes of fluids in micro-sized channels. It is widely used in diagnostics, drug development, and lab-on-a-chip technologies. Microfluidic devices can enable rapid, cost-effective, and high-throughput analysis of biological samples. Browse our peer-reviewed product directory to find the best microfluidic devices, compare products, check reviews, and get pricing directly from manufacturers.BacteriaBacteria are microorganisms that play critical roles in the environment, human health, and biotechnology. Understanding bacterial genetics, growth, and metabolism is essential for developing antibiotics, vaccines, and improving industrial processes. Browse our peer-reviewed product directory to find the best bacterial research tools, compare products, check reviews, and get pricing directly from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.

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Exploring single-cell biophysics with a simple, robust microfluidics system