Exciting additions to range of LED light sources from Olympus LEDs: The Future of Illumination

27 Jul 2008
Greg Smith
Analyst / Analytical Chemist

Product news

The flexibility of the range of light emitting diode (LED) fluorescence light sources for microscopy has been enhanced with exclusive advancements to the precisExcite® and FluoLED product series, available from Olympus. As a result, the LED light source range offers a new level of functionality and the right illumination solution for all life science fluorescence applications: From entry level single colour units to advanced high-speed multi-colour systems.

precisExcite

LED technology has proven to be an excellent alternative source of fluorescence illumination for microscopy and offers significant benefits over conventional lighting.The precisExcite represents the very latest in reflective fluorescence excitation for upright and inverted microscopes, with specially developed LED Array Modules (LAMs) as the source of illumination. The precisExcite illumination system features peltier cooling of the LAM modules. The cooling ensures highest excitation intensity, extreme stability over entire LAM lifetime with very little drop in intensity for repeatability of experiments even after years of LAM usage and completely stable illumination conditions in short- and long-time lapse experiments for best data fidelity. The precisExcite has now extended its flexibility with the introduction of four new LAMs to the portfolio of eleven mono LED array modules. These include the unique 490 nm, which is important for the fluorescence detection of the most popular fluorophore FITC, as well as 535, 565 and 585 nm LAM wavelengths. These enable it to attain intensities not possible on other LED-based fluorescence excitation systems providing peerless fluorescence microscopy with excellent capabilities for all applications. Furthermore, the LAMs are easily interchangeable and permit up to three different wavelengths to be used at once. This enables the user to select wavelengths quickly and effortlessly, and also allows for a more convenient workflow, saving valuable time when compared to mechanical wavelength switches using filter turrets or filter wheels. In addition, for a more integrated system, the precisExcite can now be fully controlled via the Olympus cell^A to cell^P software programmes, extending its functionality further still.

FluoLED
The FluoLED series is a perfect entry-level fluorescence system for educational and routine use, available in three versions to match different user needs: the FluoLED EasyBlue provides a single wavelength (480 nm) for the most cost efficient solution; the FluoLED Single provides users with the capability to use one of seven interchangeable LED cassettes from 365 nm (UV) to 630 nm (red); and the FluoLED Multifluo enables up to three of the LED cassettes to be installed and controlled at once via a three channel elctronic driver. Each model is specifically designed to fit directly to Olympus CX microscopes, and enables transmitted fluorescence observations of transparent specimens, such as bacteria and thin sections, without removing the ability to perform brightfield illumination. Broadening this application area is the ability to power LEDs by battery or solar power, ensuring that fluorescence microscopy can be conducted ‘in field’ where samples are fresh. The addition of the new FluoLED Single for the Olympus BX41 microscope now extends the FluoLED product range to clinical applications as well.

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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Spectroscopy LampsMonochromators, filters and spectroscopy lamps are available for specific applications of spectroscopy instruments. Available spectroscopy lamps include xenon, hollow cathode, ultraviolet, tungsten, halogen, mercury and deuterium.Cellular PathologyCellular Pathology deals with the microscopic analysis of tissue samples and cells. Sample preparation and processing includes fixation, staining, sectioning and slide mounting, using equipment such microtomes and cryostats. In choosing immunohistochemistry and immunocytochemistry kits, consider chromogens, staining method, antibodies, microscopes and imaging.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Exciting additions to range of LED light sources from Olympus LEDs: The Future of Illumination