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Evaluating Protein Glycosylation in Limited-Quantity Samples by HPAE-PAD
22 Aug 2013
Sarah Thomas
Associate Editor
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This application note describes HPAE-PAD methods for the evaluation of glycoprotein glycosylation using <10 µg amounts of protein. The study investigates human PSA to evaluate the potential presence of O-linked glycans and provided a detailed set of experiments to identify the N-linked glycans present on the protein.
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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.GlycobiologyGlycobiology encompasses research into the biological functions, synthesis and structures of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides. Mass spectrometers and their software are commonly used in the study of glycobiology, along with structure predicting tools, chromatography and NMR.GlycosylationGlycansGlycoproteins