Eppendorf and DASGIP Introduce Single-Use Mini Bioreactor

At this year’s Achema, held from June 18 – 22 in Frankfurt, Eppendorf and the DASGIP Information and Process Technology GmbH will show, for the first time, the new single-use mini bioreactor DASbox single-use vessel.

The development of the DASbox single-use vessel reinforces the strong synergy that benefits both DASGIP and the Eppendorf AG. “Within a very short time we were able to combine the long-standing competency of the Eppendorf AG in the field of plastics technology with our bioreactor expertise, which led to a new product”, explains Dr. Matthias Arnold, member of DASGIP management and head of the development department.

The DASbox single-use vessel is the first single-use bioreactor which was developed for the DASGIP Parallel Bioreactor Systems. As a fully instrumented mini bioreactor with a working volume of 60-250 ml, it was constructed specifically for use in combination with the DASGIP DASbox mini bioreactor system. Especially with cell culture users in mind, the advantages of single-use technology are combined with the benefits of parallel cultivation and the full functionality of industrial bioreactors. All critical process parameters such as pH, soluble oxygen and optical density can be monitored and controlled using industry standard probes.

Integrated dip tubes allow for controlled addition of liquids, sampling, as well as fully massflow controlled gas supply. The magnet coupled stirrer is only one of the examples for the uncompromising sterile technology of this single-use bioreactor. A special feature of the DASbox single-use vessel is the novel, liquid-free Peltier element temperature control and condensation. This innovation, which enables, amongst others, efficient liquid condensation from the exhaust air, represents a further milestone in the transfer of the requirements of classic bioreactor technology to single-use technology.

The DASbox single-use vessel accelerates bioprocess development. The small working volumes save precious cell material and media, and extensive cleaning and sterilization procedures are superfluous. Less time is required for installation of the bioreactor, and cross contaminations are virtually excluded. Parallel cultivation under precisely controlled conditions delivers reliable results, fast. Further, the comprehensive software functions of DASGIP DASware allow user friendly and detailed capture, saving, analysis and management of resulting data.