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Enzo Life Sciences Expands Its Epigenetics Portfolio to Include DNA Methylation Analysis

Enzo Life Sciences
22 Sept 2013

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Enzo Life Sciences is an established leader within the epigenetics market, providing pioneering Fluor de Lys™ kits for detection of histone modifications. As the field of epigenetics expands, Enzo Life Sciences is poised to maintain this leadership position by meeting the growing demand for more efficient and sensitive assay platforms for DNA methylation analysis, including conversion and detection kits.

DNA methylation is one of the most studied epigenetic modifications, both in terms of basic biology and biomarker discovery. It can alter gene expression, cell differentiation, and often results in unidirectional changes to genomic DNA. As DNA methylation is involved in so many cellular processes, errors resulting in abnormal patterns can lead to disease.

Enzo’s high-efficiency BioArray™ conversion kits for detection of 5-mC and 5-hmC modifications (Express DNA Methylation Kit, Blood & Tissue DNA Methylation Kit, and BioArray™ 5-hmC Methylation Kit) allow for resolution of single-nucleotide changes without the introduction of DNA damage. Unique features such as capacity for multiple sample inputs, rapid processing time, and high conversion efficiency rate, provide enhanced flexibility without sacrificing performance.

For researchers requiring downstream analysis products, new ELISA-based detection kits for 5-mC and 5-hmC exhibit high specificity comparable to mass spectrometry analysis and are amenable to high-throughput processing. Complementing the ELISA kits is a new qPCR-based detection platform, the BioPanel™ DNA Methylation Detection Kit for Human Pluripotent Stem Cells. This product is designed to quantify the percentage of DNA methylation in 6 gene promoters, including RAB25, NANOG, PTPN6, MGMT, GBP3 and LYST. With rapid analysis and minimal handling steps, researchers can quickly and reproducibly monitor stem cells for methylation changes that may impact the differentiation state of the cell line.

With this expanded offering of epigenetics products, Enzo Life Sciences enables scientists to achieve complete analysis of DNA methylation events. These products along with our HDAC and Sirtuin screening assays, Screen-Well® Epigenetics Library and numerous antibodies and active enzymes combine to provide a total solution for epigenetics-related research needs.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :EpigeneticsEpigenetics refers to heritable changes in gene expression. These changes are not caused by alterations to the DNA sequence. Three of the main systems responsible for the initiation of epigenetic change are DNA methylation, histone modification and non-coding RNA-associated gene silencing.DNA MethylationMethylationBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.qPCRQuantitative PCR (qPCR) is a molecular biology technique used to amplify and measure the amount of a specific DNA or RNA target. It plays a crucial role in gene expression analysis, pathogen detection, and quantifying DNA or RNA in various applications. Real-time PCR provides precise, quantitative results and is widely used in research, diagnostics, and clinical studies. Browse our peer-reviewed product directory to find the best qPCR solutions; compare products, check reviews, and get pricing directly from manufacturers.

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Enzo Life Sciences Expands Its Epigenetics Portfolio to Include DNA Methylation Analysis