Encouraging the Advancement of Synthetic Biology at iGEM 2012

30 Apr 2013
Sarah Thomas
Associate Editor

Product news

Integrated DNA Technologies (IDT), the world leader in oligonucleotide synthesis, served as one of the key sponsors for the 2012 International Genetically Engineered Machine Competition (iGEM). The competition, which encourages innovative research for the design and build of functional biological systems within living cells, saw 191 undergraduate teams from different nationalities register for the 2012 event. IDT provided the young research teams with access to their high-quality oligo products, including primers and synthetic genes, and was delighted to have 3 of their sponsored teams placed in the top 4 positions, with some impressive, novel bio-engineering models—an exciting prospect for future biological applications of the IDT product range.

April’s edition of DECODED, the international IDT customer newsletter, features the pioneering synthetic biology work of the Slovenian team (runners up in the 2012 World Championship Jamboree) and explores how their ‘Switch-it’ system acts to deliver therapeutics directly to target tissues via engineered, encapsulated cells. The sophisticated system utilizes TAL-effector domains with activator and repressor elements, and displays clever delivery and degradation capacity, developed with IDT gBlocks™ Gene Fragments. The system provides real biological potential for the delivery of gene-derived therapeutics.

To learn more about the Switch-it system please read the complete article in the April 3.2 issue of DECODED.

IDT will continue to support iGEM events, and will be offering further sponsorship opportunities for 2013 teams.

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Combinatorial ChemistryCombinatorial chemistry, also known as combichem, is a technique used in drug discovery to create libraries of structurally related compounds. A library is generated by synthesis with a chemical reactor system or by computer-based modeling of compound combinations. When undertaking combinatorial chemistry consider reagents, buffers, resins and standards.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.Genetic Engineering
Encouraging the Advancement of Synthetic Biology at iGEM 2012