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EMD Millipore Introduces the Magna ChIP-Seq<sup>TM</sup> Kit for Genome-wide Profiling of DNA-Protein Interactions

Merck
16 May 2011
Kerry Parker
CEO

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EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, today announced the launch of the Magna ChIP-Seq preparation kit designed to simplify genome-wide profiling of DNA-protein interactions by chromatin immunoprecipitation and next generation sequencing (ChIP-Seq).

“Genome-wide mapping of DNA-protein interactions using ChIP-Seq is indispensible for many gene regulation studies but the techniques can be challenging,” notes John Rosenfeld, Ph.D., manager, Chromatin Biology Group, EMD Millipore. “With the all-in-one Magna ChIP-Seq kit, this process is now simplified, enabling both experienced and new labs to more effectively leverage this powerful technique.”

In contrast to other products that support only part of the ChIP-Seq workflow, the Magna ChIP-Seq kit is a complete solution that provides a set of validated reagents for both chromatin immunoprecipitation and next generation library construction. These reagents, in combination with a detailed protocol and quality control guidelines, simplify the ChIP-Seq process to allow researchers to map interactions of histones, transcription factors, and other chromatin associated proteins on a genome-wide scale.

As successful immunoprecipitation is critical to construction of the sequencing library, the kit includes positive and negative control antibodies and a set of PCR primers that can be used as in-process controls or to verify technique. The kit has been used to construct ChIP-Seq libraries from as little as 1 ng of purified ChIP DNA, meaning that DNA segments associated with low abundance proteins can be sequenced.

For more information on Magna ChIP-Seq kit, please click 'request information' or 'article page'.

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Magna ChIP-Seq™ Chromatin Immunoprecipitation and Next Generation Sequencing Library Preparation Kit

Merck

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP-Seq has become an indispensable tool. The Magna ChIP-Seq Kit simplifies this technique by providing a validated set of reagents, easy-to-follow protocols, controls, and in process quality control guidelines to allow virtually any laboratory to conduct genome-wide analysis of DNA-protein interactions and the profiling of epigenetic marks.   •  Effective ChIP protocol simplifies production and immunoprecipitation of high quality chromatin   •  Reliable ChIP-Seq library construction from as little as 1 ng of purified ChIP DNA   •  Easy-to-follow protocols and in-process quality control guidelines help ensure successful ChIP and library construction in as little as 2 days   •  Magna ChIP A+G bead blend allows the use of antibodies from virtually any species or class   •  Enzymes and buffers provided in convenient master mix formulations streamlines library construction   •  Validate the ChIP procedure using inlcuded positive and negative control antibodies and a control primer set   •  Proven performance through construction and sequencing of genomic DNA libraries on an Illumina Genome Analyzer

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