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Eliminate Titrations and Reduce Variability in Next Gen Sequencing with new qPCR Solution

1 Jul 2010
Sarah Sarah
Marketing / Sales

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The new KAPA Library Quantification Kits available from Anachem utilise the power of Quantitative PCR (qPCR) to provide you with an accurate and sensitive method for quantifying Next Generation Sequencing (NGS) libraries. Free samples are also available on request by following the Company article page link top right of this page.

Standard methods for quantifying NGS libraries have a variety of disadvantages. Electrophoresis and spectrophotometry measure total nucleic acid concentrations, whereas optimal cluster density or template-to-bead ratio depend on the appropriate concentration of PCR-amplifiable DNA molecules. These methods also have low sensitivity, consuming nanograms of precious samples and are not suitable for high-throughput workflows.

qPCR, however, is a highly sensitive approach which uses a minimal amount of material and eliminates the need for time-consuming and expensive titrations. It specifically quantifies only PCR-competent DNA molecules and due to its high sensitivity, allows for the accurate quantification of low concentration libraries and significantly reduces cluster density variations. It can also be automated for high-throughput applications.

"Before qPCR was adopted for library quantification, cluster density was extremely variable. Implementation of the KAPA Library Quantification Kit into our sequencing workflows resulted in a significant reduction in variability across multiple libraries, negating the need for cluster amplification titration runs" - The Broad Institute, Cambridge, MA U.S.A.

The KAPA Library Quantification kits are optimized for the Illumina Genome Analyzer, Roche 454 Titanium series, and Roche 454 FLX series platforms and include defined, reliable DNA concentration standards and state-of-the-art qPCR reagents, containing a DNA polymerase engineered for SYBR® Green-based qPCR through a process of molecular evolution to provide a rapid, sensitive, and reliable method for quantifying PCR-amplifiable molecules in next-generation sequencing DNA libraries.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Eliminate Titrations and Reduce Variability in Next Gen Sequencing with new qPCR Solution