Electroporation of Nucleic Acids into Zebrafish Eggs and Embryos

Zebrafish are the perfect model for studying vertebrate development and genetics. Because the females lay large quantities of eggs, genetic analysis studies can be performed on embryos at different stages of development.

Electroporation of zebrafish eggs or in vivo electroporation of zebrafish embryos is the method of choice for the study of transgenesis and gene targeting in zebrafish at different stages of development.

BTX offers two systems for these applications: the ECM 630 exponential decay wave system is effective for electroporation of zebrafish eggs.

In addition, the ECM 630 HT system allows for the electroporation of 96 well plates in less than 1 minute.

The ECM 830 square wave pulse generator in combination with BTX Tweezertrodes is ideal for the in vivo electroporation of zebrafish embryos. The combination of low voltage, square wave pulsing, long pulse length and multiple pulse capability has made the ECM 830 most efficient for in vivo applications.

Electroporation offers a quick and easy way to introduce DNA, siRNA or antisense oligos to modify gene activities during different developmental stages of zebrafish embryos.

The BTX global technical support team will assist scientists worldwide to optimize their applications and choose the best accessories for their applications.