Efficient Pre-Concentration of Protein Solutions

24 Jan 2010
Sarah Sarah
Marketing / Sales

Product news

For laboratories looking to pre-concentrate protein solutions prior to separation (size exclusion chromatography, electrophoresis) or analysis (x-ray crystallography) the miVac sample concentrator from Genevac provides a highly efficient, cost effective alternative to traditional membrane centrifugation techniques.

Using membrane centrifugation valuable sample is often lost due to protein binding to the membrane. By comparison using a miVac sample concentrator ensures complete sample recovery even when taking proteins to very high concentrations. For protein laboratories that handle large numbers of protein samples - the miVac also offers the benefit of greatly reduced spending on consumables.

The miVac range has been designed to meet the requirements for speedy, yet careful biological sample concentration. Each miVac sample concentrator is designed to provide very high performance, precise temperature control and ease of use – all within a small benchtop footprint. miVac sample concentrators are capable of removing water and organic solvents from a variety of sample formats including tubes, microplates, and vials. Built-in stored methods for drying aqueous and alcohol-based samples improve performance and optimise concentration time.

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ElectrophoresisElectrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. Precast gels with a gradient can be used or gels can be hand cast. Samples are run alongside ladders or markers to identify the approximate size of a molecule. Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Find the best electrophoresis equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.X-ray CrystallographyX-ray crystallography is an analytical technique used to determine the arrangement of atoms in a crystal. Monochromatic x-rays are produced from a synchrotron or x-ray generator. An x-ray crystallography system uses a detector to measure the x-ray diffraction from the crystal. The information is used to generate a 3D image of the crystal.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein CrystallographyProtein crystallization is the process of crystallizing purified proteins for 3D structure analysis by x-ray crystallography. The main methods of protein crystallization include sitting drop, hanging drop and microbatch. It is important to control parameters such as pH, temperature and concentration. Following crystallization, detectors and software are used for data collection and analysis.
Efficient Pre-Concentration of Protein Solutions