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Early Access to Single-Cell Gene Expression Platform Announced

BD Medical
13 Feb 2017
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

BD (Becton, Dickinson and Company) a global medical technology company, today announced an early access program for the BD Resolve™ Single-Cell Gene Expression platform that has the flexibility to capture and analyze hundreds to tens of thousands of individual cells in a broad range of sizes and types.

The platform includes reagents that can analyze both the whole transcriptome and targeted or custom-designed gene-specific panels. When paired with the BD FACSMelody™ flow cytometer for cell sorting and enrichment, unwanted cells can be removed to both improve data accuracy and reduce sequencing costs.

“We are studying the regulation of processes in the kidney, which have customarily been challenging to do with current technologies,” said Dr. Vivek Bhalla, assistant professor of medicine (nephrology) at the Stanford University Medical Center. “Our early work with the BD Resolve platform has allowed us to sequence single cells from multiple samples at the same time, radically saving us on cost and time. The level of precision is also orders of magnitude better. It’s not an overstatement – we are advancing our work far beyond what we could do before.”

Single-cell genomics stands to offer new developments in several fields including immunology, oncology, immune-oncology and developmental biology, but conventional methods for isolating nucleic acids from an individual cell can present technical challenges and be financially limiting. Researchers are often left needing to compromise on performance, workflow simplicity and cell throughput (due to cost of sequencing).

“BD has more than 30 years of experience in single-cell analysis through its cell sorting and enrichment platforms - approaches that are a natural complement for next-generation sequencing,” said Stephen Gunstream, vice president of BD Genomics. “We have created a workflow that can enrich for viable cells of interest, improve cell-type resolution, and significantly reduce extraneous downstream costs due to unnecessary single-cell sequencing.”

The BD Resolve early access program and introduction at the Advances in Genome Biology and Technology (AGBT) conference accompanies the release of the new whole-genome protocol for the BD CLiC™ library prep instrument. This new protocol fully automates processes traditionally done manually. With the new protocol, the BD CLiC platform prepares libraries with 1/5th of the input mass and at 20 to 50 percent reduced cost per sample.

BD plans full commercial launch of the BD Resolve platform by early fall 2017.

Meet BD at the AGBT conference to learn more about the BD Resolve platform and the Difference of One:

  • Attend the BD workshop on Tuesday, Feb. 14, at Noon in the Grand Ballroom
  • Visit the BD Genomics gallery in Room #319 to see Data in New Dimensions: Convergence of Art, Genomics and Bioinformatics
  • Join events spotlighting the impact of BD Genomics technologies, including multiple symposia and posters

BD Resolve and BD CLiC platforms are currently available for research use only and not for diagnostic or therapeutic use.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Single Cell AnalysisSingle-cell analysis involves studying individual cells to gain insights into their behavior, gene expression, and function. This approach is valuable in cancer research, stem cell biology, and immunology. Explore single-cell analysis products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Single Cell GenomicsGenome SequencingGenome sequencing involves determining the complete DNA sequence of an organism's genome. It provides crucial information about genetic variations, mutations, and diseases. Advances in sequencing technologies, such as next-generation sequencing (NGS), have accelerated research in genomics, diagnostics, and personalized medicine. Explore genome sequencing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.TranscriptomicsSequencingSequencing refers to determining the order of nucleotides in a DNA or RNA molecule. This process is essential in genomics, diagnostics, and evolutionary biology. Techniques like next-generation sequencing (NGS) and Sanger sequencing provide high-throughput and precise data for analyzing genomes and transcriptomes. Browse our peer-reviewed product directory to find the best sequencing tools and reagents, compare products, check reviews, and get pricing directly from manufacturers.

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Early Access to Single-Cell Gene Expression Platform Announced