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Drug Discovery & Development News this Month

SelectScience
30 Jul 2014
Kerry Parker
CEO

Editorial article

Read about what has been happening in the Drug Discovery & Development community this month. Hear from our Guest Editor Peter Simpson, from AstraZeneca, about screening technologies for discovering lead compounds, apoptosis assays, toxicity analysis using mass spectrometry, embryoid bodies and ADME-Toxicology Methods:

Approaches to Finding Good Hit Compounds 'The Old and the New' - Part One
In this first part of this article, Peter Simpson summarizes the limits of high throughput screening for lead identification and the possible solutions to these failures.

Multiplexed Viability, Cytotoxicity and Apoptosis Assays for Cell-Based Screening
This article demonstrates the use of the CyBio CyBi® Well 384/1536 automated dispenser, with Promega MultiTox-Fluor Multiplex Cytotoxicity Assay technology, as a novel means for determining the relative number of live and dead cells in culture. The assay technology is shown to be sufficiently scalable, sensitive and robust to be multiplexed with other downstream assays to increase the quality of screening data.

Reducing Cycle Time for Quantification of Human IgG Using the Agilent Bio-Monolith Protein A HPLC Column
This application note describes the quantification of human IgG with the Agilent Bio-Monolith Protein A HPLC Column, using the Agilent 1260 Infinity Bio-inert Quaternary LC.

High-Throughput Analysis of Cytochrome P450 Inhibition in Intact Human Hepatocytes
Ultrafast methods of analysis were developed using the Agilent RapidFire High-Throughput Mass Spectrometry System, in combination with an Agilent 6490 Triple Quadrupole Mass Spectrometer, for cytochrome P450 (CYP) inhibition in human hepatocytes.

7 Ways to Improve Your ADME-Toxicology Methods
The pharmaceutical industry is always trying to find new ways to more efficiently get their small molecule products and biopharmaceuticals to market. To achieve this they must test their products for impurities and analytical compliance through stringent regulatory requirements. This article details seven different ways in which technology has been used in an ADME-toxicology laboratory.

Rapid Production and Size Assessment of Embryoid Bodies
In this application note, InSphero's GravityPLUS™ hanging drop platform provides a simple, automation-compatible solution to the production of consistently sized assay-ready embryoid bodies (EB).

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ADME-ToxicologyADME-toxicology (ADME-Tox) studies are used in pharmacology and pharmacokinetics to assess the activity/toxicity of drugs <i>in vivo</i> or <i>in vitro</i>. Find bioassays for absorption, distribution, metabolism, and excretion of drug molecules including cytotoxicity, transporter/permeability, metabolism and activity assays as well as hepatocytes and cell lines for ADME. Find the best ADME-toxicology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.High-Content ScreeningHigh-content screening (HCS), also known as high-content analysis (HCA), is a high-throughput technique used in drug discovery to identify substances that alter the phenotype of cells. HCS uses fluorescent microscopic imaging and automated image analysis to investigate cellular events such as apoptosis, cell viability, GPCR activation, oxide production, neurite outgrowth, and cell signaling. Find the best fluorescent labeling reagents, cellular assays, and high-content imaging systems in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Compound AnalysisCompound analysis is used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds have the desired effect, such as interaction with a target protein, during high throughput screening.CytotoxicityCytotoxicity assays measure the toxic effects of substances on cells, often used in drug testing and environmental studies. These tests are crucial in determining the safety of chemicals and pharmaceutical compounds. Explore cytotoxicity testing tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.ChromatographyChromatography is a powerful technique used to separate and analyze components of mixtures based on their chemical properties. It is widely used in fields like biochemistry, pharmaceuticals, and environmental analysis. By exploiting differences in the interaction of substances with a stationary phase and a mobile phase, chromatography enables precise purification and quantification of compounds. Whether you're working with complex biological samples or industrial chemicals, chromatography is essential for obtaining high-quality results. Browse our peer-reviewed product directory to find the best chromatography systems, compare products, read customer reviews, and get pricing directly from manufacturers.ADMECompound ScreeningCompound screening is a method used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds interact with the target protein during screening and could therefore be carried forward in the drug development process.

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