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Double-emulsion droplet sorting: Strategies and applications for multiomic profiling

SelectScience
26 Aug 2020
Georgina Wynne Hughes
Editorial Assistant

Expert insights

Kara Brower, Ph.D. candidate at Stanford University, and Dr. Marie Mikkelsen, co-founder and CTO of Samplix
Kara Brower, Ph.D. candidate at Stanford University, and Dr. Marie Mikkelsen, co-founder and CTO of Samplix

Droplet microfluidics has made a large impact in many different applications including the analysis of DNA variants, secretory biomolecules, and diverse cell types. However, to enrich individual variants of interest, researchers face challenges with high-throughput screening and isolation of these emulsion droplets, based on phenotypic expression. Integrated strategies that combine the sensitivity and precision of cell sorting with downstream single-cell multiomic analysis are now being used to address these challenges.

In this webinar, discover how gel-encapsulated microdroplets can be analyzed by high-throughput droplet sorting for genomic, proteomic, and metabolomic profiling for a diverse range of applications.

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Key learning objectives:

  • Discover how DNA variants, biomolecules, and various cell types can be successfully encapsulated within double-emulsion droplets
  • Understand how this droplet technology can be used to analyze genomic regions edited using CRISPR-Cas9 and to analyze large DNA alterations in cancer and neurological diseases
  • Learn how cell sorting can be used for high-throughput analysis and precise isolation of droplet microreactors for multiomic profiling

Certificate of attendance

All webinar participants can request a certificate of attendance and a learning outcomes summary document for continuing education purposes.

Attend the webinar on Wednesday, September 9, at:

  • 16:00 BST
  • 17:00 CEST
  • 08:00 PDT
  • 11:00 EDT

Register for this webinar here>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.MetabolomicsMetabolomics is the study of small metabolites (the intermediates and products of metabolism). It involves the identification and quantification of cellular metabolites using analytical technologies such as GC, HPLC, NMR, and LC/MS.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.GenomicsGenomics is the study of genomes, focusing on the sequencing, analysis, and interpretation of genetic material. It is key in understanding genetic diseases, evolutionary biology, and personalized medicine. Techniques like next-generation sequencing (NGS) are commonly used in genomics research. Browse our peer-reviewed product directory to find the best genomics tools, compare products, check reviews, and get pricing directly from manufacturers.CRISPRCRISPR technology enables precise editing of genes, allowing scientists to modify DNA at specific locations. This revolutionary tool is used in genetic research, drug development, and gene therapy. CRISPR has applications in agriculture, disease treatment, and creating genetically modified organisms (GMOs). Explore CRISPR solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.MicrofluidicsMicrofluidics is the science of manipulating small volumes of fluids in micro-sized channels. It is widely used in diagnostics, drug development, and lab-on-a-chip technologies. Microfluidic devices can enable rapid, cost-effective, and high-throughput analysis of biological samples. Browse our peer-reviewed product directory to find the best microfluidic devices, compare products, check reviews, and get pricing directly from manufacturers.

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Double-emulsion droplet sorting: Strategies and applications for multiomic profiling