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Don’t Miss Our Free Webinar on Quantitative MicroRNA Expression Analysis in Challenging Samples

SelectScience

08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST, May 20, 2015

11 May 2015
Sarah Thomas
Associate Editor

Expert insights

Discover how to approach the detection microRNAs in challenging samples in this free, interactive SelectScience webinar on entitled Quantitative MicroRNA Expression Analysis in Tissues and Body Fluids: Whole Genome Profiling Using Small RNA Sequencing, Followed by qPCR Based Validation. You won't want to miss this exciting presentation - register now!

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a key role in gene expression regulation. They have critical functions in various biological processes and their involvement in disease biology is well documented. The accurate and reliable detection of microRNAs in challenging samples, such as formalin fixed paraffin embedded (FFPE) tissue samples and body fluids including serum, plasma, urine and others, is of interest for the identification of new biomarkers for cancer and other diseases.

Several measurement platforms have been developed to determine relative miRNA abundance in biological samples using different technologies, such as small RNA sequencing, reverse transcription-quantitative PCR (RT-qPCR) and hybridization.

During this webinar, Dr. Ariane De Ganck, Product Manager, Transcriptome Services at Biogazelle, will discuss:

  • The combined approach of whole genome microRNA profiling using small RNA sequencing and targeted microRNA validation.
  • The workflow, starting from sample preparation to microRNA measurement, data processing and interpretation.
  • Case studies for microRNA expression analysis in challenging samples, including FFPE tissue and body fluids.
  • How the QIAGEN miScript PCR system can be used for targeted microRNA measurement.

The Q&A session of the webinar will also provide the opportunity for you to ask Dr. De Ganck any questions you have about the techniques and case studies discussed.

Register now to secure your place for this exciting online event. If you can't make the live event, don’t worry – we will send all registrants a link to the on-demand version after the event.

08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST, May 20, 2015

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :RNARNA is a nucleic acid that plays a key role in gene expression and protein synthesis. It serves as a messenger between DNA and ribosomes, carrying genetic instructions to produce proteins. Advances in RNA-based therapies, such as mRNA vaccines and gene editing, have revolutionized treatment strategies for genetic disorders and infectious diseases. Explore RNA research tools and therapies in our peer-reviewed product directory; compare products, check customer reviews, and get pricing directly from manufacturers.FFPEFormalin-fixed paraffin-embedded (FFPE) tissues are commonly used in medical research, particularly in histology and cancer studies. The preservation process allows long-term storage of tissue samples for later analysis. Explore FFPE research products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.RNA-SeqRNA sequencing (RNA-seq) is a next-generation sequencing technique used to analyze the transcriptome, providing insights into gene expression and regulation. This method is essential for genomics, disease research, and personalized medicine. Explore RNA-seq tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.EpigeneticsEpigenetics refers to heritable changes in gene expression. These changes are not caused by alterations to the DNA sequence. Three of the main systems responsible for the initiation of epigenetic change are DNA methylation, histone modification and non-coding RNA-associated gene silencing.

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