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DNA 5'末端加磷一步到位！TaKaRa DNA Kination Kit
10 Jul 2007Meng Ye
Student / Graduate
Product news
TaKaRa DNA Kination Kit是对非磷酸化DNA片段（单链及双链)，如：PCR产物、Linker、Adaptor、Primer等的5'末端进行磷酸化的试剂盒。试剂盒中含有T4 Polynucleotide Kinase和ATP，与TaKaRa公司独自开发的5'末端高效磷酸化反应液相结合，可以对DNA片段（单链及双链)的5'末端进行高效磷酸化。
试剂盒中的pSIMPLE-18 EcoR V/BAP Vector是TaKaRa公司根据pUC 18载体改建而成的平滑末端去磷酸化载体，本载体消去了pUC 18载体上的多克隆酶切位点，但仍保持着β-半乳糖苷酶的正常表达，进行DNA克隆时仍可以利用α-互补性进行蓝白菌落筛选。由于消去了多克隆酶切位点，克隆后的DNA片段将无法用载体上的酶切位点切下，此时需要在DNA片段的两端导入合适的酶切位点，使用导入的DNA片段上的酶切位点进行DNA切断时，酶切反应将不会受到原有载体上其它多克隆酶切位点上的限制酶影响，可以大大提高酶切效率，增加亚克隆的成功率。
试剂盒中还含有Control Insert 3以及高效连接液Ligation Mix，可以进行Control实验。而高效连接液Ligation Mix可以在极短时间内（5分钟左右）完成连接反应，且其连接液可以直接用于细菌转化，大大方便了克隆实验。
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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical GeneticsMolecular Genetics covers the analysis of hereditary genetic disease and chromosomal abnormalities. Genetics can be analysed using DNA, RNA, and protein microarrays, PCR, RT PCR and DNA sequencing. Genetic equipment includes genetic workstations, thermal cyclers, cooling blocks and electrophoresis products. Diagnostic kits are used for DNA / RNA extraction and purification.