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Dionex Introduces AFC-3000 Automated Fraction Collector

Thermo Fisher Scientific
23 Nov 2009
Sarah Sarah
Marketing / Sales

Product news

Dionex has released the AFC-3000 Automated Fraction Collector, a reliable and highly flexible fraction collector, ideally suited for preparative reversed-phase or normal-phase HPLC as well as purification of proteins, peptides, and nucleic acids.

The AFC-3000 manages analytical and semipreparative to preparative flow rates, and collects fractions into a large variety of commonly used sample formats, including 96-well plates for small volume to bottles for large volume fraction collection. A funnel rack directs virtually unlimited volumes into any bottle or reactor.

The AFC-3000 is compatible with all solvents, buffers, acids, and bases, and can be equipped with an optional grounded drop former for safe operation with normal-phase eluents. The fraction collector supports flow rates up to 150 mL/min, and has the smallest possible carryover due to minimized delay volume between the valve and drop former. An optional kit for improved performance at low flow rates is available. The AFC-3000 is ideally suited for working with the comprehensive Chromeleon® Chromatography Data System fraction collection features.

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Dionex Introduces AFC-3000 Automated Fraction Collector