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Digital imaging basics for optical microscopy
Join us on Tuesday, December 8, for this masterclass on understanding the link between biology and digital image data
4 Dec 2020
Cameron Smith-Craig
Pharma and Applied Sciences Editor
Expert insights
In this SelectScience webinar, we present the basics of microscopy imaging with a digital camera to educate users on how to improve both their image and data quality.
Using examples in widefield fluorescence imaging, Takeo Ogama, Olympus Corporation of the Americas, will cover the link between the biology and observed digital image data, the factor effects on signal intensity, causes of background noise and how to minimize them, trade-off factors that make an impact on image and data quality, and best practices for image acquisition.
Register hereKey learning objectives
- Basics of microscopy digital image acquisition
- Understanding the link between biology and digital image data
- How to achieve optimal signal-to-noise ratio while maximizing the signal, minimizing the background, and keeping the sample healthy
- Best practices for fluorescence image acquisition
Who should attend?
Biologists who use an optical microscope with a camera for fluorescence imaging.
Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary for continuing education purposes.
This webinar will run on Tuesday, December 8, at:
- 16:00 GMT
- 17:00 CET
- 08:00 PST
- 11:00 EST
Register for this webinar here>>
SelectScience runs more than 10 webinars a month across various scientific topics, discover more of our webinars here>>
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Fluorescence SpectroscopyFluorometers and spectrofluorometers (also called fluorescence spectrometers) are used to measure the intensity and wavelength of fluorescent light emitted from a sample after excitation by illumination. Spectrofluorometers utilize monochromators to select the desired wavelengths, whereas filter fluorometers employ a set of filters. Spectrofluorometers for measuring steady-state fluorescence and lifetime fluorescence (or time-resolved fluorescence) are available, as well as fluorescence microscopes and microplate readers. Find the best fluorescence spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Light MicroscopyLight microscopes or optical microscopes are used to visualize microscale objects under magnification, including cells, clinical specimens and materials. Lab equipment for light microscopy includes confocal microscopes, fluorescence microscopes, zoom and stereo microscopes. Microscope slides and imaging reagents are available for visualizing samples, as well as various microscope stages and incubators for large or temperature-sensitive samples. Find the best light microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Electron MicroscopyElectron microscopes (EM) are used to create high-resolution images of samples at the nanoscale by means of an accelerated beam of electrons as a source of illumination. Types of electron microscope include scanning electron microscopes (SEM), transmission electron microscopes (TEM), scanning transmission electron microscopes (STEM) and cryo-electron microscopes. Focused ion beam (FIB) microscopes are useful for modifying or milling a sample surface with nanometer precision, as well as imaging. Find the best electron microscopes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Digital MicroscopyDigital microscopy involves using digital cameras and sensors to capture high-resolution images of samples for analysis. It offers enhanced imaging capabilities compared to traditional optical microscopy and is widely used in biological and material science research. Explore digital microscopy systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Cell ImagingCell imaging can be achieved using a number of techniques including confocal microscopy, transmission electron microscopy, atomic force microscopy, and light sheet microscopy.Fluorescence MicroscopyFluorescence microscopy has become an essential tool in biology, as well as in materials science. The application of many fluorochromes has made it possible to identify cells and sub-microscopic cellular components with a high degree of specificity. Using multiple fluorescence labels, different probes can simultaneously identify several target molecules.ImagingImaging techniques are essential for obtaining visual representations of samples to understand structures, processes, and function in biological, chemical, and physical research. These tools range from traditional light microscopy to advanced imaging modalities like MRI and electron microscopy, providing researchers with valuable data for diagnostics, drug discovery, and material analysis. Explore imaging solutions in our peer-reviewed product directory to compare products, check reviews, and get pricing directly from manufacturers.3D Imaging3D imaging technologies allow for the visualization and analysis of three-dimensional structures at high resolution. These systems are used in fields like molecular biology, material science, and medical diagnostics. 3D imaging can be applied to visualize cells, tissues, and organs, providing valuable insights into their structure and function. Browse our peer-reviewed product directory to find the best 3D imaging solutions, compare products, check reviews, and get pricing directly from manufacturers.Live Cell ImagingLive cell imaging is the study of living cells using microscopes and high-content imaging systems. This technique provides in-depth insight into fast and complex biological processes, by allowing dynamic imaging of living cells instead of acquiring an individual image at a single point in time.MicroscopyMicroscopy is a technique used to observe small objects in detail, from cells to materials, using light or electron microscopes. It enables researchers to examine structures with high resolution, aiding in fields such as biology, medicine, and materials science. With advanced microscopy techniques, scientists can gain insights into cellular processes, tissue structures, and material properties. Explore the best microscopy solutions in our peer-reviewed product directory, compare products, read customer reviews, and get pricing directly from manufacturers.Digital ImagingDigital imaging is a technique that captures images of biological and chemical samples in high resolution, often using advanced cameras and sensors. This technology is crucial in microscopy, diagnostics, and quality control. Explore digital imaging systems in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.