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Developing an assay platform for screening and characterization of antibody-drug conjugates

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Don't miss this webinar to find out how researchers at LifeArc are using ALK-specific antibodies as a mechanism of directly targeting ALK-expressing neuroblastoma cells

7 Jun 2020
Sophie Ball
Publishing / Media

Expert insights

Dr. Rachel Forfar, Senior Scientist, LifeArc

Oncogenic mutations of anaplastic lymphoma kinase (ALK), a receptor tyrosine kinase, have been identified in a number of carcinomas. More recently, overexpression of mutated ALK has been linked to neuroblastoma cancer cells and is associated with poor prognosis in young patients. While current therapies target intracellular signaling cascades, ALK mutant variants demonstrate kinase inhibitor resistance, and therefore new mechanisms to target these cancer cells are crucial for better treatment.

Join this webinar to discover how researchers at LifeArc are investigating a novel approach whereby cytotoxic agents are conjugated to ALK-specific antibodies as a mechanism of directly targeting ALK-expressing neuroblastoma cells.

Register Now

Join this webinar to get an overview of:

  • LifeArc cell-based assays that utilize advanced flow cytometry and live-cell analysis
  • An assessment of cell cytotoxicity using secondary antibodies conjugated to potent toxins
  • The challenges of screening, from initial stages with hybridoma supernatants, through to candidate antibodies and towards effectively identifying antibodies with the desired properties of an antibody-drug conjugate (ADC)

Attend the live webinar on Thursday, June 11, at:

  • 16:00 BST
  • 17:00 CEST
  • 08:00 PDT
  • 11:00 EDT

Register for this webinar here>>

SelectScience runs 3-4 webinars a month across various scientific topics, discover more of our upcoming webinars>>

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Gel Doc / Image AnalysisGel documentation (gel doc) or gel imaging systems are used for the analysis of proteins, antibodies and nucleic acid immobilized in polyacrylamide or agarose gels, membranes or microarrays. Explore a range of a gel imaging systems, densitometers, scanners, transilluminators or UV lamp + CCD cameras for your image analysis solutions. Colorimetric, fluorescent and/or radioisotopic samples can be visualized and documented for further analysis. See gel doc / Image analysis software for quantitative 1D and 2D analysis of your samples. Find the best gel doc / image analysis products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell SignalingEngineered AntibodiesEngineered antibodies are modified to enhance specificity, stability, and functionality for therapeutic and diagnostic applications. These antibodies are central to immunotherapy and biomarker detection. Compare engineered antibody solutions in our directory to find top products and reviews.Cancer ResearchAlthough cancer is often referred to as a single condition, it actually consists of more than 100 different diseases. Microscopy, mass spectrometry, high throughput sequencing and flow cytometry are some of the most common techniques employed in cancer research labs.

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