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Detect Your Unseen Proteins
Duolink Amplified Secondary Antibody Detection
26 Oct 2016
Lynsey Forsyth
Post Doc / Research Fellow
Product news
The vast majority of proteins interact with other proteins for proper biological activity. Duolink enables you to detect, quantify and visualize protein-protein interactions, single target protein expression levels, and post translational modification events. This technology outperforms traditional protein detection methods by visualizing proteins in their native state through combining the selectivity of antibodies and sensitivity of nucleic acid amplification.
- Visualize individual interactions without having to overexpress proteins
- Detect and quantify weak and transient interactions at endogenous levels
- Gain high specificity with dual binding of primary antibodies
- Single molecule sensitivity due to signal amplification
- Analyze using standard immunofluorescence instruments
- Amenable to high throughput cell-based screens
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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.High-Throughput ScreeningHigh-throughput screening (HTS) is an automated drug discovery technique for identification of active compounds against a compound library. Use HTS readers and integrated assay preparation / analysis workstations to screen your compounds. Identify active compounds against various HTS libraries, including membranes, proteins and peptides and HTS cell lines. Find the best high-throughput screening products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.AntibodiesFluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.High ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.