Core-Shell HPLC/UHPLC Technology for Proteins and Peptides

Phenomenex Inc. has introduced Aeris™ – core-shell HPLC/UHPLC columns specially designed for the analysis of proteins and peptides. These new high-performance columns deliver high resolution and peak capacities, producing sub-2 micron results on any LC instrument. With virtually no bleed, Aeris columns are highly compatible with MS.

Aeris columns are offered in wide- and small-pore configurations. Aeris WIDEPORE 3.6-micron core-shell columns are optimized for the separation of intact proteins and polypeptides, and are available in three selectivity’s – XB-C18, XB-C8 and C4. Because the 3.6-micron core-shell particles produce lower backpressures than traditional media, longer or coupled columns can be used to increase resolving power on both HPLC and UHPLC instruments.

Aeris PEPTIDE 3.6-micron and 1.7-micron columns, designed with a small pore size, are recommended for the separation of low molecular weight peptides and for peptide mapping. The novel XB-C18 stationary phase provides the ideal surface chemistry for resolving peptides. Aeris PEPTIDE 3.6-micron particles offer high performance at backpressures compatible with both HPLC and UHPLC instruments. On UHPLC systems, the 1.7-micron Aeris particles can deliver higher peak capacities than fully porous sub-2 micron columns.

“The new Aeris columns are ‘plug and play,’ requiring very little work in method transfer,” comments Kari Kelly, brand manager for Phenomenex. “With a little method optimization, users can achieve even higher resolution and better performance, and our PhenoLogix support team is ready to help customers with their switch to Aeris.”