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Convenient Pre-packed Formats for High Purity Separation of Histidine-tagged Proteins

Cytiva
28 Feb 2012
Roger Wayman
Administrator / Office Personnel

Product news

GE Healthcare has launched a range of chromatography media for the high purity separation of histidine-tagged proteins. This new range includes: HiTrap™ TALON crude, His SpinTrap™ TALON microspin, His MultiTrap™ TALON and His GraviTrap™ TALON pre-packed with TALON Superflow™ media for rapid screening, preparative purification and easy scale-up of histidine-tagged proteins. Samples derived from a variety of expression systems can be readily purified with minimal sample preparation time and no additional equipment costs.

TALON Superflow is a cobalt-based medium designed for the purification of histidine-tagged recombinant proteins by immobilized metal affinity chromatography (IMAC). Compared to nickel-charged media, cobalt-based media offers enhanced selectivity and yield purity for histidine-tagged proteins. Additionally, lower concentrations of imidazole are required during purification which better preserves the structure and function of sensitive histidine-tagged proteins.

Key features of the TALON Superflow medium and pre-packed formats:

• Suitable for IMAC purification of histidine-tagged proteins when Ni2+ is not the optimal choice of metal ion

• High yield purity in a single step using a mild purification process which preserves structure and function of sensitive histidine-tagged proteins

• Pre-packed HiTrap TALON crude 1 ml and 5 ml columns and His GraviTrap TALON gravity flow columns allow direct loading of unclarified samples to minimize labor and protein degradation

• His SpinTrap TALON spin columns and His MultiTrap TALON 96-well plates enable screening with highly reproducible column-to-column and well-to-well results.

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Ion ChromatographyIon chromatography, also known as ion exchange chromatography, is a high-performance liquid chromatographic technique used for the separation and identification of ions or polar molecules in a sample, including proteins, nucleotides and amino acids. Equipment includes ion exchange columns, ion exclusion columns, ion chromatography systems, pumps, and detectors. Find the best ion chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Preparative ChromatographyPreparative chromatography equipment is used for purification purposes, separating and collecting compounds eluted from a mixture which can then be subsequently used for further purposes. This can be done in large quantities or small. Preparative chromatography systems are usually low to medium pressure liquid chromatographs that are semi-automated. Explore a range of preparative columns and fraction collectors. Find the best preparative chromatography equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Histidine-Tagged Proteins

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Convenient Pre-packed Formats for High Purity Separation of Histidine-tagged Proteins